Essential Genetics 6th Edition by Daniel Hartl

Exercise 21

DNA from 100 unrelated people was digested with the restriction enzyme Hind III, and the resulting fragments were separated and probed with a sequence for a particular gene. Four fragment lengths that hybridized with the probe were observed, which were of size 5.7, 6.0, 6.2, and 6.5 kb. Each fragment defines a different restriction-fragment allele. The accompanying illustration shows the gel patterns observed among the sample. The number of individuals with each gel pattern is shown across the top. Estimate the allele frequencies of the four restriction-fragment alleles.