Molecular Biology of the Gene 7th Edition by Richard Losick, James Watson, Michael Levine, Tamara Baker, Alexander Gann

Exercise 14

With respect to the wild-type sequence below, identify the mutation in each altered sequence as one of the following types: missense mutation, nonsense mutation, frameshift mutation, reverse mutation, intragenic suppressor mutation, or intergenic suppressor mutation. For each altered sequence, more than one answer is possible. This DNA sequence encodes the last amino acids of a protein that is 270 amino acids long. The first three bolded base pairs indicate the frame and include the coding region. Inserted nucleotides are italicized.
Wild type:
5?... GCT AAGTATTGCTCAAGATTAGGATGATAAATAACTGG3?
3?... CGA TTCATAACGAGTTCTAATCCTACTATTTATTGACC5?
A. Altered sequence 1:
5?... GCT AAGTATTGCTCAACCGATTAGGATGATAAATAACTGG3? 3?... CGA TTCATAACGAGTT GGCTAATCCTACTATTTATTGACC5?
B. Altered sequence 2 - changing altered sequence 1:
5?... GCT AAGTATTGCTCA CC GATTAGGATGATAAATAACTGG3?
3?... CGA TTCATAACGAGT GGCTAATCCTACTATTTATTGACC5?
C. Altered sequence 3- changing altered sequence 1:
5?... GCT AAGTATTGCTCCCGATTAGGATGATAAATAACTGG3?
3?... CGA TTCATAACGAGGGCTAATCCTACTATTTATTGACC5?