Molecular Biology of the Gene 7th Edition by Richard Losick, James Watson, Michael Levine, Tamara Baker, Alexander Gann

Exercise 2

You are cloning a gene from Candida albicans that you want to express in E. coli for purification. You want the E. coli cells to make a protein with the exact amino acid sequence as in Candida albicans. How could you mutate the DNA sequence of your gene of interest to ensure that the 5?-CUG-3? codon codes for serine for instead of leucine?