Molecular Biology Of The Cell 6th Edition by Bruce Alberts, Alexander Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter

Exercise 3

Antibodies that bind to specific proteins are import­ ant tools for defining the locations of molecules in cells. The sensitivity of the primary antibody-the antibody that reacts with the target molecule-is often enhanced by using labeled secondary antibodies that bind to it. What are the advantages and disadvantages of using secondary antibodies that carry fluorescent tags versus those that carry bound enzymes?