Biomedical Engineering: Bridging Medicine and Technology 1st Edition by Veronique Tran,Mark Saltzman

Exercise 12

A circular piece of DNA (e.g., a plasmid) was cleaved using three different restriction enzymes. The cut sizes of the fragments of DNA were detected by gel electrophoresis. The results of the restriction enzyme treatments are shown here (note that 1 kb = 1000 base pairs).
a. What is the length (in kb) of the circular DNA?
b. Draw a map of the circular DNA (including the restriction sites for HindIII, EcoRI, BamHI) and determine the distance (in kb) between all the restriction sites on the circular DNA.