
Bioprocess Engineering 2nd Edition by Fikret Kargi,Michael Shuler
Edition 2ISBN: 9780130819086
Bioprocess Engineering 2nd Edition by Fikret Kargi,Michael Shuler
Edition 2ISBN: 9780130819086 Exercise 8
Consider the use of gel chromatography to separate two proteins A and B. The partition coef-
ficient
for A is 0.5 and for B is 0.15.
the void volume in the column, is
Vi, the void volume within the gel particles, is
The total volume of the column is 60
The flow rate of elutant is
Ignoring dispersion and other effects, how long
will it take for A to exit the column? How long for B?
ficient






will it take for A to exit the column? How long for B?
Explanation
The fundamental equa...
Bioprocess Engineering 2nd Edition by Fikret Kargi,Michael Shuler
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