Bioprocess Engineering 2nd Edition by Fikret Kargi,Michael Shuler

Exercise 5

Consider the following data for E. coli B/r-pDW17 grown in a minimal medium supple-
mented with amino acids. Estimate and R. Compare the stability of this system to one
with a glucose-minimal medium (Example 14.2).
Example 14.2.
The data in Table 14.4 were obtained for E. coli B/r-pDW17 at two different dilution rates in
glucose-limited chemostats. The average plasmid copy number for pDW17 is about 40 to 50
copies per cell. About 12of the total protein synthesized is due to the plasmid. The proteins
are retained intracellularly in soluble form. Use these data to estimate