Bioprocess Engineering 2nd Edition by Fikret Kargi,Michael Shuler

Question

Bioprocess Engineering 2nd Edition by Fikret Kargi,Michael Shuler

Edition 2ISBN: 9780130819086

Bioprocess Engineering 2nd Edition by Fikret Kargi,Michael Shuler

Edition 2ISBN: 9780130819086

Exercise 10

Assume you scale up from $Assume you scale up from cells/ml of 100% plasmid-containing cells to cells/ml, at which point overproduction of the target protein is induced. You harvest six hours after induction. The value of P is 0.0005. Before induction After induction What is the fraction of plasmid- containing cells at induction? What is the fraction of plasmid-containing cells at harvest?$ cells/ml of 100% plasmid-containing cells to $Assume you scale up from cells/ml of 100% plasmid-containing cells to cells/ml, at which point overproduction of the target protein is induced. You harvest six hours after induction. The value of P is 0.0005. Before induction After induction What is the fraction of plasmid- containing cells at induction? What is the fraction of plasmid-containing cells at harvest?$ cells/ml, at which point overproduction of the target protein is induced. $Assume you scale up from cells/ml of 100% plasmid-containing cells to cells/ml, at which point overproduction of the target protein is induced. You harvest six hours after induction. The value of P is 0.0005. Before induction After induction What is the fraction of plasmid- containing cells at induction? What is the fraction of plasmid-containing cells at harvest?$ You harvest six hours after induction. The value of P is 0.0005. Before induction $Assume you scale up from cells/ml of 100% plasmid-containing cells to cells/ml, at which point overproduction of the target protein is induced. You harvest six hours after induction. The value of P is 0.0005. Before induction After induction What is the fraction of plasmid- containing cells at induction? What is the fraction of plasmid-containing cells at harvest?$ $Assume you scale up from cells/ml of 100% plasmid-containing cells to cells/ml, at which point overproduction of the target protein is induced. You harvest six hours after induction. The value of P is 0.0005. Before induction After induction What is the fraction of plasmid- containing cells at induction? What is the fraction of plasmid-containing cells at harvest?$ After induction $Assume you scale up from cells/ml of 100% plasmid-containing cells to cells/ml, at which point overproduction of the target protein is induced. You harvest six hours after induction. The value of P is 0.0005. Before induction After induction What is the fraction of plasmid- containing cells at induction? What is the fraction of plasmid-containing cells at harvest?$ What is the fraction of plasmid-
containing cells at induction? What is the fraction of plasmid-containing cells at harvest?

Explanation

Verified

a) 1 × 10 ³ (1 × 10 10 )(1)
= 10 13 ce...