Question 1

Commercial blood culture medium is not recommended for recovery of Mycoplasma spp. due to

Accepted Answer

A)  the small volume of blood used. 
B)  the slow growth of the organisms. 
C)  inhibition of the organisms by SPS. 
D)  the fact that the organisms do not survive in liquid medium. 
A)  the small volume of blood used. 
B)  the slow growth of the organisms. 
C)  inhibition of the organisms by SPS. 
D)  the fact that the organisms do not survive in liquid medium. C

Question 2

A 20-year-old male has blood drawn as part of his physical to play football. He tells the physician he has had a cough and sore throat for the last three weeks. In the hematology laboratory, the hematologist runs the blood sample and the results show severe anemia. To ensure the result is accurate, she warms the blood for 10 minutes and retests it. This time the red blood cell count is within the normal range. This type of reaction may be seen after infection with

Accepted Answer

A)  Acholeplasma laidlawii. 
B)  Mycoplasma hominis. 
C)  M. pneumoniae. 
D)  M. orale. 
A)  Acholeplasma laidlawii. 
B)  Mycoplasma hominis. 
C)  M. pneumoniae. 
D)  M. orale. C

Question 3

Due to the difficulty in culturing for Mycoplasma pneumoniae, the best method for detection is

Accepted Answer

A)  complement fixation. 
B)  enzyme immunoassay. 
C)  polymerase chain reaction. 
D)  detection of cold agglutinins. 
A)  complement fixation. 
B)  enzyme immunoassay. 
C)  polymerase chain reaction. 
D)  detection of cold agglutinins. C

Question 4

Which of the following organisms is associated with nongonococcal urethritis?

Accepted Answer

A)  Mycoplasma hominis 
B)  M. genitalium 
C)  Ureaplasma urealyticum 
D)  b and c 
E)  All of the above 
A)  Mycoplasma hominis 
B)  M. genitalium 
C)  Ureaplasma urealyticum 
D)  b and c 
E)  All of the above

Question 5

Medium incorporated with serum, arginine, penicillin, and a pH indicator is used in the growth and detection of

Accepted Answer

A)  Ureaplasma urealyticum. 
B)  Mycoplasma pneumoniae. 
C)  M. hominis. 
D)  all of the above. 
E)  b and c. 
A)  Ureaplasma urealyticum. 
B)  Mycoplasma pneumoniae. 
C)  M. hominis. 
D)  all of the above. 
E)  b and c.

Question 6

Ureaplasma spp. and Mycoplasma spp. are unique in their requirement for ________________ to maintain their cell membrane.

Accepted Answer

A)  arginine 
B)  sterols 
C)  glucose 
D)  urea 
A)  arginine 
B)  sterols 
C)  glucose 
D)  urea

Question 7

Which of the following organisms may cause bacteremia after genitourinary manipulations?

Accepted Answer

A)  Mycoplasma pneumoniae 
B)  M. hominis 
C)  M. genitalium 
D)  Ureaplasma urealyticum 
A)  Mycoplasma pneumoniae 
B)  M. hominis 
C)  M. genitalium 
D)  Ureaplasma urealyticum

Question 8

Serologic diagnosis of infection with Mycoplasma pneumoniae can be made by

Accepted Answer

A)  a single serum. 
B)  paired sera, drawn two weeks apart. 
C)  paired sera, drawn two months apart. 
D)  a single serum in conjunction with type O Rh-negative erythrocytes at 4°C. 
A)  a single serum. 
B)  paired sera, drawn two weeks apart. 
C)  paired sera, drawn two months apart. 
D)  a single serum in conjunction with type O Rh-negative erythrocytes at 4°C.

Question 9

Serological tests are not available for diagnostic purposes for

Accepted Answer

A)  Mycoplasma pneumoniae. 
B)  M. hominis. 
C)  Ureaplasma urealyticum. 
D)  b and c. 
E)  a and b. 
A)  Mycoplasma pneumoniae. 
B)  M. hominis. 
C)  Ureaplasma urealyticum. 
D)  b and c. 
E)  a and b.

Question 10

What is the method of choice for performance of susceptibility for Mycoplasma spp. and Ureaplasma spp.?

Accepted Answer

A)  Agar dilution 
B)  Disk diffusion 
C)  Broth dilution 
D)  None of the above 
A)  Agar dilution 
B)  Disk diffusion 
C)  Broth dilution 
D)  None of the above

Question 11

Which of the following virulence factors accounts for Mycoplasma pneumoniae's ability to injure the respiratory mucosal epithelial cells?

Accepted Answer

A)  The specialized attachment tip 
B)  Adhesions produced 
C)  Production of hydrogen peroxide 
D)  Development of cold agglutinins 
A)  The specialized attachment tip 
B)  Adhesions produced 
C)  Production of hydrogen peroxide 
D)  Development of cold agglutinins

Question 12

Polymerase chain reaction is ideal for direct detection of

Accepted Answer

A)  Mycoplasma hominis. 
B)  M. pneumoniae. 
C)  Ureaplasma urealyticum. 
D)  all of the above. 
A)  Mycoplasma hominis. 
B)  M. pneumoniae. 
C)  Ureaplasma urealyticum. 
D)  all of the above.

Question 13

The production of cold agglutinins with infection by Mycoplasma pneumoniae is due to

Accepted Answer

A)  activation of T and B cells. 
B)  adhesions produced by the organism. 
C)  the production of hydrogen peroxide. 
D)  an autoimmue response. 
A)  activation of T and B cells. 
B)  adhesions produced by the organism. 
C)  the production of hydrogen peroxide. 
D)  an autoimmue response.

Question 14

SP-4 medium is inoculated with a specimen. After incubation, it turns yellow due to the presence of a member of the class Mollicutes. The original specimen was probably

Accepted Answer

A)  bronchoalveolar lavage. 
B)  placenta. 
C)  blood. 
D)  kidney stone. 
A)  bronchoalveolar lavage. 
B)  placenta. 
C)  blood. 
D)  kidney stone.

Question 15

Given the colony on A-8 agar provided in the image, what was the original source of the specimen cultured?
  Source: CDC and Dr. Francis Forrester

Accepted Answer

A)  Sputum 
B)  Blood 
C)  Urine 
D)  Amniotic fluid 
A)  Sputum 
B)  Blood 
C)  Urine 
D)  Amniotic fluid

Question 16

Cultural methods have proven to be insensitive for the recovery of

Accepted Answer

A)  Ureaplasma urealyticum. 
B)  Mycoplasma pneumoniae. 
C)  M. hominis. 
D)  b and c. 
A)  Ureaplasma urealyticum. 
B)  Mycoplasma pneumoniae. 
C)  M. hominis. 
D)  b and c.

Question 17

Mycoplasma spp. and Ureaplasma spp. differ from other bacteria in that they

Accepted Answer

A)  permanently lack a cell wall. 
B)  demonstrate the ability to gain and lose their cell wall. 
C)  can be Gram stained when they possess a cell wall. 
D)  b and c. 
A)  permanently lack a cell wall. 
B)  demonstrate the ability to gain and lose their cell wall. 
C)  can be Gram stained when they possess a cell wall. 
D)  b and c.

Question 18

A placenta culture from a febrile 37-year-old female at delivery yields Ureaplasma spp. The physician will treat the new mother with

Accepted Answer

A)  erythromycin. 
B)  clindamycin. 
C)  penicillin. 
D)  trimethoprim/sulfamethoxazole. 
A)  erythromycin. 
B)  clindamycin. 
C)  penicillin. 
D)  trimethoprim/sulfamethoxazole.

Question 19

The presence of kidney stones made of struvite and calcium phosphates can be caused due to metabolic processes or infection with

Accepted Answer

A)  Mycoplasma hominis. 
B)  M. genitalium. 
C)  M. fermentans. 
D)  Ureaplasma urealyticum. 
A)  Mycoplasma hominis. 
B)  M. genitalium. 
C)  M. fermentans. 
D)  Ureaplasma urealyticum.

Question 20

Ureaplasma spp. can be presumptively identified on A-8 agar by its

Accepted Answer

A)  ability to hydrolyze urea. 
B)  granular colony. 
C)  ability to hydrolyze arginine. 
D)  a and b. 
E)  b and c. 
A)  ability to hydrolyze urea. 
B)  granular colony. 
C)  ability to hydrolyze arginine. 
D)  a and b. 
E)  b and c.

Exam 28: Mycoplasma and Ureaplasma

Commercial blood culture medium is not recommended for recovery of Mycoplasma spp. due to

Due to the difficulty in culturing for Mycoplasma pneumoniae, the best method for detection is

Which of the following organisms is associated with nongonococcal urethritis?

Medium incorporated with serum, arginine, penicillin, and a pH indicator is used in the growth and detection of

Ureaplasma spp. and Mycoplasma spp. are unique in their requirement for ________________ to maintain their cell membrane.

Which of the following organisms may cause bacteremia after genitourinary manipulations?

Serologic diagnosis of infection with Mycoplasma pneumoniae can be made by

Serological tests are not available for diagnostic purposes for

What is the method of choice for performance of susceptibility for Mycoplasma spp. and Ureaplasma spp.?

Which of the following virulence factors accounts for Mycoplasma pneumoniae's ability to injure the respiratory mucosal epithelial cells?

Polymerase chain reaction is ideal for direct detection of

The production of cold agglutinins with infection by Mycoplasma pneumoniae is due to

SP-4 medium is inoculated with a specimen. After incubation, it turns yellow due to the presence of a member of the class Mollicutes. The original specimen was probably

Given the colony on A-8 agar provided in the image, what was the original source of the specimen cultured? Source: CDC and Dr. Francis Forrester

Cultural methods have proven to be insensitive for the recovery of

Mycoplasma spp. and Ureaplasma spp. differ from other bacteria in that they

A placenta culture from a febrile 37-year-old female at delivery yields Ureaplasma spp. The physician will treat the new mother with

The presence of kidney stones made of struvite and calcium phosphates can be caused due to metabolic processes or infection with

Ureaplasma spp. can be presumptively identified on A-8 agar by its

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