Question 1

Which of the following describes alpha hemolysis on sheep blood agar?

Accepted Answer

A)  Complete clearing around the colonies of the red blood cells in the medium 
B)  Partial hemolysis of the red blood cells in the medium appearing as a green discoloration of the medium 
C)  Partial hemolysis of the red blood cells in the medium appearing as a slight clearing around the colonies 
D)  No hemolysis of the red blood cells in the medium; therefore no change in the color of the medium 
A)  Complete clearing around the colonies of the red blood cells in the medium 
B)  Partial hemolysis of the red blood cells in the medium appearing as a green discoloration of the medium 
C)  Partial hemolysis of the red blood cells in the medium appearing as a slight clearing around the colonies 
D)  No hemolysis of the red blood cells in the medium; therefore no change in the color of the medium B

Question 2

Which of the following organisms can be routinely detected by culture and nucleic acid testing?

Accepted Answer

A)  Gardnerella vaginalis 
B)  Neisseria gonorrhoeae 
C)  Chlamydia trachomatis 
D)  Both b and c 
A)  Gardnerella vaginalis 
B)  Neisseria gonorrhoeae 
C)  Chlamydia trachomatis 
D)  Both b and c D

Question 3

Which of the following is an agar disk diffusion method of testing for antimicrobial susceptibility?

Accepted Answer

A)  Microdilution 
B)  Macrodilution 
C)  Kirby-Bauer method 
D)  MBC 
A)  Microdilution 
B)  Macrodilution 
C)  Kirby-Bauer method 
D)  MBC C

Question 4

Which of the following specimen types for culture can be collected in a clean,nonsterile container?

Accepted Answer

A)  Urine 
B)  Stool 
C)  Cerebrospinal fluid 
D)  Blood 
A)  Urine 
B)  Stool 
C)  Cerebrospinal fluid 
D)  Blood

Question 5

Which of the following is a sterilization procedure that uses moist heat and how is this method effective in killing bacterial spores?

Accepted Answer

A)  Filtration; filters all microbes larger than the filter pore size 
B)  Boiling; kills all microorganisms and spores at 100° C (boiling) 
C)  Hot-air chamber; kills all microorganisms and bacterial spores at 171° C for at least 1 hour 
D)  Autoclaving; kills all microorganisms and bacterial spores at 121° C for 15 minutes at 15 lb above atmospheric pressure 
A)  Filtration; filters all microbes larger than the filter pore size 
B)  Boiling; kills all microorganisms and spores at 100° C (boiling) 
C)  Hot-air chamber; kills all microorganisms and bacterial spores at 171° C for at least 1 hour 
D)  Autoclaving; kills all microorganisms and bacterial spores at 121° C for 15 minutes at 15 lb above atmospheric pressure

Question 6

A microbiologist observes colonies on Hektoen enteric agar (HE)that appear green.Which of the following organisms should the microbiologist suspect?

Accepted Answer

A)  Salmonella spp. 
B)  Escherichia coli 
C)  Proteus spp. 
D)  Shigella spp. 
A)  Salmonella spp. 
B)  Escherichia coli 
C)  Proteus spp. 
D)  Shigella spp.

Question 7

What is the color of gram-negative bacterial cells after the decolorization step?

Accepted Answer

A)  Purple/blue 
B)  Red/pink 
C)  Iodine yellow 
D)  Colorless 
A)  Purple/blue 
B)  Red/pink 
C)  Iodine yellow 
D)  Colorless

Question 8

The microbiologist uses TSI and LIA agars to rule out stool pathogens,Salmonella and Shigella spp.The reactions observed are TSI: K/A,H2S+ and LIA: R/A.What should the technologist do next?

Accepted Answer

A)  Report Salmonella spp. recovered 
B)  Report Shigella spp. recovered 
C)  Report normal stool flora 
D)  Repeat all testing 
A)  Report Salmonella spp. recovered 
B)  Report Shigella spp. recovered 
C)  Report normal stool flora 
D)  Repeat all testing

Question 9

Which of the following is the "cold" method for staining acid-fast bacteria?

Accepted Answer

A)  Ziehl-Neelsen 
B)  Kinyoun 
C)  Fluorochrome 
D)  All of the above 
A)  Ziehl-Neelsen 
B)  Kinyoun 
C)  Fluorochrome 
D)  All of the above

Question 10

Which of the following is a selective and differential medium for the recovery of gram-positive organisms?

Accepted Answer

A)  Columbia colistin-nalidixic acid agar with blood (CN
A)  
B)  Eosin-methylene blue agar (EM
B)  
C)  MacConkey agar (MA
C)  
D)  Xylose-lysine desoxycholate agar (XL
D)  
A)  Columbia colistin-nalidixic acid agar with blood (CN
A)  
B)  Eosin-methylene blue agar (EM
B)  
C)  MacConkey agar (MA
C)  
D)  Xylose-lysine desoxycholate agar (XL
D)

Question 11

Chocolate agar contains all of the following except:

Accepted Answer

A)  antibiotics. 
B)  X factor. 
C)  V factor. 
D)  lysed blood. 
A)  antibiotics. 
B)  X factor. 
C)  V factor. 
D)  lysed blood.

Question 12

If a urine culture was planted using a 0.001-mL loop and 75 colonies were observed,what is the colony-forming units per millimeter of urine (CFU/mL)?

Accepted Answer

A)  75 CFU/mL 
B)  750 CFU/mL 
C)  7500 CFU/mL 
D)  75,000 CFU/mL 
A)  75 CFU/mL 
B)  750 CFU/mL 
C)  7500 CFU/mL 
D)  75,000 CFU/mL

Question 13

Which of the following bacteria are typically stained using the acid-fast technique?

Accepted Answer

A)  Mycobacteria 
B)  Staphylococci 
C)  Streptococci 
D)  Escherichia coli 
A)  Mycobacteria 
B)  Staphylococci 
C)  Streptococci 
D)  Escherichia coli

Question 14

The technique of killing or destroying all microorganisms including bacterial spores (endospores)is called:

Accepted Answer

A)  antisepsis. 
B)  sterilization. 
C)  physical disinfection. 
D)  chemical disinfection. 
A)  antisepsis. 
B)  sterilization. 
C)  physical disinfection. 
D)  chemical disinfection.

Question 15

Which of the following is the feeding and reproductive stage of protozoa and what consistency of stool would you usually find them in?

Accepted Answer

A)  Cysts; loose or watery stool 
B)  Cysts; formed stool 
C)  Trophozoites; loose or watery stool 
D)  Trophozoites; formed stool 
A)  Cysts; loose or watery stool 
B)  Cysts; formed stool 
C)  Trophozoites; loose or watery stool 
D)  Trophozoites; formed stool

Question 16

A common gram-negative organism found in blood is:

Accepted Answer

A)  Staphylococcus aureus. 
B)  Escherichia coli. 
C)  coagulase-negative staphylococci. 
D)  Streptococcus pneumoniae. 
A)  Staphylococcus aureus. 
B)  Escherichia coli. 
C)  coagulase-negative staphylococci. 
D)  Streptococcus pneumoniae.

Question 17

Which of the following media contain sodium thiosulfate and ferric ammonium citrate for the detection of hydrogen sulfide production?

Accepted Answer

A)  Triple sugar iron agar (TSI) 
B)  Lysine iron agar (LI
A)  
C)  Xylose-lysine-desoxycholate agar (XL
D)  
D)  All of the above 
A)  Triple sugar iron agar (TSI) 
B)  Lysine iron agar (LI
A)  
C)  Xylose-lysine-desoxycholate agar (XL
D)  
D)  All of the above

Question 18

Which of the following is the least desirable specimen for fungal cultures?

Accepted Answer

A)  Hair 
B)  Skin 
C)  Sterile body fluids 
D)  Swabs 
A)  Hair 
B)  Skin 
C)  Sterile body fluids 
D)  Swabs

Question 19

The technologist uses TSI and LIA agars to rule out stool pathogens,Salmonella and Shigella spp.The reactions observed are TSI: K/A H2S+ and LIA: K/K.What should the technologist do next?

Accepted Answer

A)  Report Salmonella spp. recovered 
B)  Report Shigella spp. recovered 
C)  Do further biochemical and serological testing to confirm Salmonella spp. 
D)  Repeat all testing 
A)  Report Salmonella spp. recovered 
B)  Report Shigella spp. recovered 
C)  Do further biochemical and serological testing to confirm Salmonella spp. 
D)  Repeat all testing

Question 20

Sending appropriately collected specimens to the laboratory before antibiotic therapy is initiated is important because:

Accepted Answer

A)  pathogenic bacteria could be hard to recover because of the effects of the antibiotic. 
B)  collection of specimens from around the wound area can result in contamination of the culture. 
C)  collection of a sputum specimen contaminated with saliva could yield a culture result that reflects normal mouth flora. 
D)  All of the above are correct. 
A)  pathogenic bacteria could be hard to recover because of the effects of the antibiotic. 
B)  collection of specimens from around the wound area can result in contamination of the culture. 
C)  collection of a sputum specimen contaminated with saliva could yield a culture result that reflects normal mouth flora. 
D)  All of the above are correct.

Which of the following describes alpha hemolysis on sheep blood agar?

Which of the following organisms can be routinely detected by culture and nucleic acid testing?

Which of the following is an agar disk diffusion method of testing for antimicrobial susceptibility?

Which of the following specimen types for culture can be collected in a clean,nonsterile container?

Which of the following is a sterilization procedure that uses moist heat and how is this method effective in killing bacterial spores?

A microbiologist observes colonies on Hektoen enteric agar (HE)that appear green.Which of the following organisms should the microbiologist suspect?

What is the color of gram-negative bacterial cells after the decolorization step?

The microbiologist uses TSI and LIA agars to rule out stool pathogens,Salmonella and Shigella spp.The reactions observed are TSI: K/A,H2S+ and LIA: R/A.What should the technologist do next?

Which of the following is the "cold" method for staining acid-fast bacteria?

Which of the following is a selective and differential medium for the recovery of gram-positive organisms?

Chocolate agar contains all of the following except:

If a urine culture was planted using a 0.001-mL loop and 75 colonies were observed,what is the colony-forming units per millimeter of urine (CFU/mL)?

Which of the following bacteria are typically stained using the acid-fast technique?

The technique of killing or destroying all microorganisms including bacterial spores (endospores)is called:

Which of the following is the feeding and reproductive stage of protozoa and what consistency of stool would you usually find them in?

A common gram-negative organism found in blood is:

Which of the following media contain sodium thiosulfate and ferric ammonium citrate for the detection of hydrogen sulfide production?

Which of the following is the least desirable specimen for fungal cultures?

The technologist uses TSI and LIA agars to rule out stool pathogens,Salmonella and Shigella spp.The reactions observed are TSI: K/A H2S+ and LIA: K/K.What should the technologist do next?

Sending appropriately collected specimens to the laboratory before antibiotic therapy is initiated is important because:

Fundamentals of the Clinical Laboratory

Safety in the Clinical Laboratory

Quality Assessment and Quality Control in the Clinical Laboratory

Phlebotomy: Collecting and Processing Blood

The Microscope

Systems of Measurement, laboratory Equipment, and Reagents

Laboratory Mathematics and Solution Preparation

Basic and New Techniques in the Clinical Laboratory

Delivery of Laboratory Testing From Point of Care to Total Automation

Introduction to Clinical Chemistry

Principles and Practice of Clinical Hematology

Introduction to Hemostasis

Renal Physiology and Urinalysis

Examination of Body Fluids and Miscellaneous Specimens

Immunology and Serology

Immunohematology and Transfusion Medicine

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Exam 15: Introduction to Microbiology

Which of the following describes alpha hemolysis on sheep blood agar?

Which of the following organisms can be routinely detected by culture and nucleic acid testing?

Which of the following is an agar disk diffusion method of testing for antimicrobial susceptibility?

Which of the following specimen types for culture can be collected in a clean,nonsterile container?

Which of the following is a sterilization procedure that uses moist heat and how is this method effective in killing bacterial spores?

A microbiologist observes colonies on Hektoen enteric agar (HE)that appear green.Which of the following organisms should the microbiologist suspect?

What is the color of gram-negative bacterial cells after the decolorization step?

The microbiologist uses TSI and LIA agars to rule out stool pathogens,Salmonella and Shigella spp.The reactions observed are TSI: K/A,H2S+ and LIA: R/A.What should the technologist do next?

Which of the following is the "cold" method for staining acid-fast bacteria?

Which of the following is a selective and differential medium for the recovery of gram-positive organisms?

Chocolate agar contains all of the following except:

If a urine culture was planted using a 0.001-mL loop and 75 colonies were observed,what is the colony-forming units per millimeter of urine (CFU/mL)?

Which of the following bacteria are typically stained using the acid-fast technique?

The technique of killing or destroying all microorganisms including bacterial spores (endospores)is called:

Which of the following is the feeding and reproductive stage of protozoa and what consistency of stool would you usually find them in?

A common gram-negative organism found in blood is:

Which of the following media contain sodium thiosulfate and ferric ammonium citrate for the detection of hydrogen sulfide production?

Which of the following is the least desirable specimen for fungal cultures?

The technologist uses TSI and LIA agars to rule out stool pathogens,Salmonella and Shigella spp.The reactions observed are TSI: K/A H2S+ and LIA: K/K.What should the technologist do next?

Sending appropriately collected specimens to the laboratory before antibiotic therapy is initiated is important because:

Fundamentals of the Clinical Laboratory

Safety in the Clinical Laboratory

Quality Assessment and Quality Control in the Clinical Laboratory

Phlebotomy: Collecting and Processing Blood

The Microscope

Systems of Measurement, laboratory Equipment, and Reagents

Laboratory Mathematics and Solution Preparation

Basic and New Techniques in the Clinical Laboratory

Delivery of Laboratory Testing From Point of Care to Total Automation

Introduction to Clinical Chemistry

Principles and Practice of Clinical Hematology

Introduction to Hemostasis

Renal Physiology and Urinalysis

Examination of Body Fluids and Miscellaneous Specimens

Immunology and Serology

Immunohematology and Transfusion Medicine

Filters