Deck 3: The Microscope

Question 1

If your ocular lens is 10×, and you are using your 45× objective lens to view a slide, the total magnification is _ .

A)250×
B)55×
C)90×
D)450×

Answer 1

D

Question 2

As you examine the tissue on your slide, you switch to a greater magnification to increase the size of the cells you see. You _ the light intensity to see better at the greater magnification.

A)make no adjustment to
B)decrease
C)increase

Answer 2

C

Question 3

When you want to study a slide under the microscope, you place it on the .

A)arm
B)stage
C)head
D)base

Answer 3

B

Question 4

You have learned that the objective lens magnifies the object on the slide, producing the image, and that the ocular lens magnifies that image, forming the image.

A)ocular, objective
B)objective, real
C)real, virtual
D)virtual, ocular

Question 5

When studying a slide in a microscope, begin focusing with the - power objective lens in place.

A)highest
B)midrange
C)lowest

Question 6

As you increase the total magnification of the specimen, the working distance _.

A)increases
B)decreases
C)remains the same

Question 7

The is a substage lens that concentrates light on the specimen.

A)light source
B)condenser
C)iris diaphragm lever
D)objective lens

Question 8

With the higher power objective lenses, use only this knob to focus.

A)fine adjustment knob
B)iris diaphragm lever
C)course adjustment knob

Question 9

The microscope field is the .

A)platform on which the slide rests
B)area you see through the lenses of the microscope
C)space near the microscope where you sit
D)area in which you place the microscope

Question 10

If the diameter of your microscope field is 1 mm at low power (10×)and your ocular lens is 10×, then what is the diameter of your field when you switch to the 50× objective lens?

A)0)02 mm
B)0)2 mm
C)0)002 mm
D)2 mm

Question 11

As total magnification increases, the depth of field .

A)increases
B)decreases
C)remains the same

Question 12

This is the formula for calculating the diameter of an unknown microscope field.

A)diameter of field A × total magnification of field A = diameter of field B × total magnification of field B
B)diameter of field A + total magnification of field A = diameter of field B + total magnification of field B
C)diameter of field A + diameter of field B = total magnification of field A + total magnification of field B
D)diameter of field A × diameter of field B = total magnification of field A × total magnification of field B

Question 13

Microscope specimens have depth, as well as length and width. If you are focused on the middle layer of cells and wish to see the top layer of cells clearly, you should move the .

A)slide toward or away from you
B)slide to the left or right
C)lens further away from the slide
D)lens closer to the slide

Question 14

You prepare a wet mount of your own cheek cells and place it under the microscope, but find that you cannot see any detail of the cells. What can you do to make details more visible?

A)increase light intensity
B)prepare another slide and stain with methylene blue
C)view at a higher power
D)view at a lower power

Question 15

The ability to discriminate two close objects as separate is called .

A)parfocal
B)working distance
C)virtual image
D)resolution (or resolving power)

Question 16

You are looking at very lightly stained cells under the microscope, and you want to increase the contrast so that you can see them "better." You _ the iris diaphragm to the light.

A)open, increase
B)open, dim
C)close, increase
D)close, dim

Question 17

You begin your observations using the lens and the adjustment knob.

A)highest- power, fine
B)lowest- power, coarse
C)midrange- power, fine
D)midrange- power, coarse

Question 18

The of the microscope carries three or four objective lenses.

A)arm
B)base
C)ocular
D)nosepiece

Question 19

To transport a microscope, hold it _ .

A)horizontally, with one hand supporting the objective lenses
B)cradled in both arms
C)upright, with one hand on its arm and the other hand supporting its base
D)close to your body with one arm and cover the top of it with your other arm

Question 20

The working distance for a 10× objective lens is .

A)much less than the working distance for the 100× objective lens
B)a little bit less than the working distance for the 100× objective lens
C)a little bit greater than the working distance for the 100× objective lens
D)much greater than the working distance for the 100× objective lens

Question 21

As you increase the total magnification of the specimen, the size of the field _.

A)increases
B)decreases
C)remains the same

Question 22

The depth of field .

A)depends on the tissue being studied
B)depends on the thickness of the slide
C)is the depth of the slide that is clearly in focus

Question 23

You are looking at a slide of three crossed threads. Yellow is on the bottom, blue is in the middle, and red is on the top. When you rotate the adjustment knob forward (away from you)on your microscope, the stage rises. You move the adjustment knob to focus on the middle thread. As you rotate the adjustment knob forward, which color thread will come into focus next?

A)red
B)blue
C)yellow

Question 24

If a circular object seen in your low- power field (diameter 1 mm)occupies about 1/4 of the diameter of the field, the object's diameter is about .

A)2)5 µm
B)0)25 µm
C)250 µm
D)25 µm

Question 25

The total magnification of an object can be found by _ .

A)dividing the power of the objective lens by the power of the ocular lens
B)multiplying the power of the objective lens by the number of objective lenses on your microscope
C)multiplying the power of the objective lens by the power of the ocular lens
D)adding the power of the objective lens and the power of the ocular lens