Exam 3: The Microscope

You have learned that the objective lens magnifies the object on the slide, producing the________ image, and that the ocular lens magnifies that image, forming the________ image.

If the diameter of your microscope field is 1 mm at low power (10×)and your ocular lens is 10×, then what is the diameter of your field when you switch to the 50× objective lens?

The ________is a substage lens that concentrates light on the specimen.

You are looking at a slide of three crossed threads. Yellow is on the bottom, blue is in the middle, and red is on the top. When you rotate the adjustment knob forward (away from you)on your microscope, the stage rises. You move the adjustment knob to focus on the middle thread. As you rotate the adjustment knob forward, which color thread will come into focus next?

You are looking at very lightly stained cells under the microscope, and you want to increase the contrast so that you can see them "better." You _________ the iris diaphragm to ________the light.

If your ocular lens is 10×, and you are using your 45× objective lens to view a slide, the total magnification is________ .

The microscope field is the ________.

As total magnification increases, the depth of field________ .

The working distance for a 10× objective lens is________ .

The________ of the microscope carries three or four objective lenses.

You begin your observations using the________ lens and the ________adjustment knob.

You prepare a wet mount of your own cheek cells and place it under the microscope, but find that you cannot see any detail of the cells. What can you do to make details more visible?

As you increase the total magnification of the specimen, the size of the field _________.

This is the formula for calculating the diameter of an unknown microscope field.

With the higher power objective lenses, use only this knob to focus.

Microscope specimens have depth, as well as length and width. If you are focused on the middle layer of cells and wish to see the top layer of cells clearly, you should move the________ .

When you want to study a slide under the microscope, you place it on the________ .

The total magnification of an object can be found by _________ .

As you examine the tissue on your slide, you switch to a greater magnification to increase the size of the cells you see. You _________ the light intensity to see better at the greater magnification.

When studying a slide in a microscope, begin focusing with the ________- power objective lens in place.