Deck 18: Recombinant Dna Technology

Question 1

Which of the following is an example of a palindromic DNA sequence?

A) 5' - ATCGAC - 3' 3' - TAGCAG - 5'
B) 5' - ATCATC - 3' 3' - ATCATC - 5'
C) 5' - GCCGCC - 3' 3' - CGGCGG - 5'
D) 5' - CTGCAG - 3' 3' - GACGTC - 5'

Answer 1

D

Question 2

What occurs during the annealing stage of a PCR reaction?

A) The DNA strands separate
B) The DNA polymerase copies the template DNA
C) The primers bind to the ends of the template DNA
D) The reaction stops
E) All of the answers are correct

Answer 2

C

Question 3

What is a DNA library?

A) A collection of vectors, each containing a fragment of the original genome
B) A complete sequence of the genetic material in an organism
C) An electronic file that may be used for additional genetic analysis
D) A collection of mRNA from a given cell
E) None of the answers are correct

Answer 3

A

Question 4

Which of the following is needed for a PCR reaction?

A) Template DNA
B) Primers
C) DNA polymerase
D) Deoxynucleotide triphosphates
E) All of the answers are correct

Question 5

DNA ligase is needed in a cloning experiment for what reason?

A) To proofread the vector for mistakes
B) To reestablish the stable sugar-phosphate backbone of the DNA molecule
C) To allow the vector to enter into the cell
D) As a selectable marker

Question 6

RT-PCR is used for which of the following?

A) Conduct a PCR reaction without the use of primers
B) Generate cDNA
C) Quantify RNA in a cell
D) Nonspecific DNA amplification
E) None of the answers are correct

Question 7

A plasmid that contains separate origins of replication for two different species is called a .

A) R factor
B) Expression vector
C) Viral vector
D) Shuttle vector
E) None of the answers are correct

Question 8

What is the origin of restriction endonucleases?

A) They are part of DNA repair mechanisms in eukaryotic cells
B) They are a defense mechanism against viruses in bacteria
C) They are replication enzymes of yeast
D) They are transposable elements of Drosophila

Question 9

Which of the following would a competent cell be able to do?

A) Resist transfection by a viral vector
B) Repair DNA strands without the aid of DNA lygase
C) Utilize transposons as a vector
D) Take up DNA from the external environment
E) None of the answers are correct

Question 10

cDNA is made using what as the starting material?

A) Plasmid vectors
B) Viral DNA
C) Chromosomal DNA
D) RNA

Question 11

Small circular pieces of bacterial DNA that are used as vectors in cloning experiments are called .

A) Phages
B) Viruses
C) Plasmids
D) Chromosomes
E) None of the answers are correct

Question 12

Restriction endonucleases are specific in the DNA sequences that they recognize.

Question 13

Which of the following would contain both vector DNA and chromosomal DNA?

A) Recircularized vector
B) Hybrid vector
C) Both vectors
D) Neither vector

Question 14

In a cloning experiment, you use a vector that contains a lacZ gene as a selectable marker. If the competent cells are grown on X-Gal and IPTG, which colonies would contain chromosomal DNA?

A) The white colonies
B) The blue colonies
C) Half the total colonies
D) None of the colonies

Question 15

A cDNA library differs from a DNA library in which way?

A) It consists solely of RNA molecules
B) It contains many copies of the gene of interest
C) It contains only coding sequences, not introns
D) It is typically 10-100 times the size of a DNA library

Question 16

The presence of a specific plasmid in a bacterial cell may be determined if the plasmid .

A) Is exceptionally large
B) Is an expression vector
C) Contains pieces of a viral genome
D) Contains a selectable marker
E) None of the answers are correct

Question 17

Which of the following is correct regarding the first cloning experiment?

A) It utilized the EcoR1 restriction enzyme
B) The vector was a plasmid called pSC101
C) The vector had a selectable marker for tetracycline resistance
D) The process involved the cloning of kanamycinR gene
E) All of the answers are correct

Question 18

Which of the following individual(s) developed the process of the polymerase chain reaction?

A) Watson and Crick
B) McClintock
C) Mullis
D) Pauling
E) None of the answers are correct

Question 19

Restriction mapping may be used to .

A) Determine the precise location of a gene on a chromosome
B) Determine the location of restriction sites in relation to one another
C) Generate sub clones
D) None of the answers are correct

Question 20

What is the purpose of RNaseH in the making of cDNA?

A) It copies the RNA into a complementary DNA strand
B) It reforms the sugar-phosphate backbone
C) It generates short RNAs that are used as primers
D) It proofreads the cDNA for errors

Question 21

The use of fluorescent-labeled dideoxynucleotides is a characteristic of what type of sequencing?

A) Sequencing ladders
B) Automated sequencing
C) Base-specific DNA cleavage
D) All of the answers are correct

Question 22

A special vector that is designed to clone the coding sequence of a gene correctly is called a structural
vector.

Question 23

Southern blotting may be used for which of the following?

A) Identify gene families
B) Detect the number of copies of a gene in the genome
C) Identify deletions
D) Identify homologous genes in different species
E) All of the answers are correct

Question 24

A vector is a small segment of DNA that a gene of interest is inserted into for cloning.

Question 25

Which of the following techniques may be used to determine DNA-protein interactions?

A) Western blotting
B) PCR
C) DNAse I foot printing
D) Restriction enzyme analysis

Question 26

Which of the following is NOT a stage of a PCR reaction?

A) DNA is heated to a temperature at which the hydrogen bonds holding the strands break.
B) The primers bind to the complementary sequences on the template DNA.
C) DNA gyrase enzyme relieves the supercoiling ahead of the replication fork.
D) The DNA polymerase synthesizes a new strand of DNA that is complementary to the template DNA.

Question 27

Which of the following is used to identify a specific DNA sequence in a library?

A) Western blotting
B) Northern blotting
C) Southern blotting
D) Eastern blotting

Question 28

The isolation and copying of a gene, usually in large quantities is called gene cloning.

Question 29

R factors typically contain genes for antibiotic resistance.

Question 30

Which of the following is used to identify a specific protein in a library?

A) Western blotting
B) Northern blotting
C) Southern blotting
D) Eastern blotting

Question 31

Which of the following is used to identify a specific RNA in a library?

A) Western blotting
B) Northern blotting
C) Southern blotting
D) Eastern blotting

Question 32

How might a probe for a colony hybridization experiment be obtained?

A) By using a known copy of the gene
B) By using a similar gene from a closely related species
C) Using antibodies that detect the clones expressing the protein
D) By using small fragments of the protein to generate probes
E) All of the answers are possible

Question 33

Transfection is when a bacterial vector is taken up into the host cell.

Question 34

Dideoxy nucleotides are used in which of the following techniques?

A) RT-PCR
B) DNA sequencing
C) DNAse I foot printing
D) Restriction mapping
E) Gene cloning

Question 35

Antibodies can be used as a probe for which of the following techniques?

A) Western blotting
B) Northern blotting
C) Southern blotting
D) Eastern blotting

Question 36

The DNA Pol III enzyme can use RNA to make a DNA strand.

Question 37

conditions are essential in a Southern blot experiment if the researcher wants to identify the closest DNA match to the probe.

A) High stringency
B) Low stringency

Question 38

The polymerase used in PCR reactions is derived from the thermophile organism Thermus aquaticus.

Question 39

Molecular biologists use restriction enzymes to amplify a specific section of DNA within the genome.

Question 40

Since cDNA is made from RNA, it lacks introns and contains only the coding sequence for the
functional protein.

Question 41

Cloned genes may be mutated outside of a cell by a process called in vitro site-directed mutagensis.

Question 42

A dideoxy nucleotide is missing an -OH group at the 2' end.

Question 43

The use of a radioactive probe to screen a DNA library for a gene of interest is called colony
hybridization.