Question 1

Which of the following is used to identify a specific RNA in a library?

Accepted Answer

A)  Western blotting 
B)  Northern blotting 
C)  Southern blotting 
D)  Eastern blotting 
A)  Western blotting 
B)  Northern blotting 
C)  Southern blotting 
D)  Eastern blotting B

Question 2

Which of the following is NOT a stage of a PCR reaction?

Accepted Answer

A)  DNA is heated to a temperature at which the hydrogen bonds holding the strands break. 
B)  The primers bind to the complementary sequences on the template DNA. 
C)  DNA gyrase enzyme relieves the supercoiling ahead of the replication fork. 
D)  The DNA polymerase synthesizes a new strand of DNA that is complementary to the template DNA. 
A)  DNA is heated to a temperature at which the hydrogen bonds holding the strands break. 
B)  The primers bind to the complementary sequences on the template DNA. 
C)  DNA gyrase enzyme relieves the supercoiling ahead of the replication fork. 
D)  The DNA polymerase synthesizes a new strand of DNA that is complementary to the template DNA. C

Question 3

What is the purpose of RNaseH in the making of cDNA?

Accepted Answer

A)  It copies the RNA into a complementary DNA strand 
B)  It reforms the sugar-phosphate backbone 
C)  It generates short RNAs that are used as primers 
D)  It proofreads the cDNA for errors 
A)  It copies the RNA into a complementary DNA strand 
B)  It reforms the sugar-phosphate backbone 
C)  It generates short RNAs that are used as primers 
D)  It proofreads the cDNA for errors C

Question 4

Which of the following would contain both vector DNA and chromosomal DNA?

Accepted Answer

A)  Recircularized vector 
B)  Hybrid vector 
C)  Both vectors 
D)  Neither vector 
A)  Recircularized vector 
B)  Hybrid vector 
C)  Both vectors 
D)  Neither vector

Question 5

How might a probe for a colony hybridization experiment be obtained?

Accepted Answer

A)  By using a known copy of the gene 
B)  By using a similar gene from a closely related species 
C)  Using antibodies that detect the clones expressing the protein 
D)  By using small fragments of the protein to generate probes 
E)  All of the answers are possible 
A)  By using a known copy of the gene 
B)  By using a similar gene from a closely related species 
C)  Using antibodies that detect the clones expressing the protein 
D)  By using small fragments of the protein to generate probes 
E)  All of the answers are possible

Question 6

The use of fluorescent-labeled dideoxynucleotides is a characteristic of what type of sequencing?

Accepted Answer

A)  Sequencing ladders 
B)  Automated sequencing 
C)  Base-specific DNA cleavage 
D)  All of the answers are correct 
A)  Sequencing ladders 
B)  Automated sequencing 
C)  Base-specific DNA cleavage 
D)  All of the answers are correct

Question 7

Which of the following is correct regarding the first cloning experiment?

Accepted Answer

A)  It utilized the EcoR1 restriction enzyme 
B)  The vector was a plasmid called pSC101 
C)  The vector had a selectable marker for tetracycline resistance 
D)  The process involved the cloning of kanamycinR gene 
E)  All of the answers are correct 
A)  It utilized the EcoR1 restriction enzyme 
B)  The vector was a plasmid called pSC101 
C)  The vector had a selectable marker for tetracycline resistance 
D)  The process involved the cloning of kanamycinR gene 
E)  All of the answers are correct

Question 8

Which of the following techniques may be used to determine DNA-protein interactions?

Accepted Answer

A)  Western blotting 
B)  PCR 
C)  DNAse I foot printing 
D)  Restriction enzyme analysis 
A)  Western blotting 
B)  PCR 
C)  DNAse I foot printing 
D)  Restriction enzyme analysis

Question 9

Small circular pieces of bacterial DNA that are used as vectors in cloning experiments are called .

Accepted Answer

A)  Phages 
B)  Viruses 
C)  Plasmids 
D)  Chromosomes 
E)  None of the answers are correct 
A)  Phages 
B)  Viruses 
C)  Plasmids 
D)  Chromosomes 
E)  None of the answers are correct

Question 10

Antibodies can be used as a probe for which of the following techniques?

Accepted Answer

A)  Western blotting 
B)  Northern blotting 
C)  Southern blotting 
D)  Eastern blotting 
A)  Western blotting 
B)  Northern blotting 
C)  Southern blotting 
D)  Eastern blotting

Question 11

DNA ligase is needed in a cloning experiment for what reason?

Accepted Answer

A)  To proofread the vector for mistakes 
B)  To reestablish the stable sugar-phosphate backbone of the DNA molecule 
C)  To allow the vector to enter into the cell 
D)  As a selectable marker 
A)  To proofread the vector for mistakes 
B)  To reestablish the stable sugar-phosphate backbone of the DNA molecule 
C)  To allow the vector to enter into the cell 
D)  As a selectable marker

Question 12

Dideoxy nucleotides are used in which of the following techniques?

Accepted Answer

A)  RT-PCR 
B)  DNA sequencing 
C)  DNAse I foot printing 
D)  Restriction mapping 
E)  Gene cloning 
A)  RT-PCR 
B)  DNA sequencing 
C)  DNAse I foot printing 
D)  Restriction mapping 
E)  Gene cloning

Question 13

What occurs during the annealing stage of a PCR reaction?

Accepted Answer

A)  The DNA strands separate 
B)  The DNA polymerase copies the template DNA 
C)  The primers bind to the ends of the template DNA 
D)  The reaction stops 
E)  All of the answers are correct 
A)  The DNA strands separate 
B)  The DNA polymerase copies the template DNA 
C)  The primers bind to the ends of the template DNA 
D)  The reaction stops 
E)  All of the answers are correct

Question 14

The DNA Pol III enzyme can use RNA to make a DNA strand.

Accepted Answer

A) True 
 B)False

Question 15

The presence of a specific plasmid in a bacterial cell may be determined if the plasmid .

Accepted Answer

A)  Is exceptionally large 
B)  Is an expression vector 
C)  Contains pieces of a viral genome 
D)  Contains a selectable marker 
E)  None of the answers are correct 
A)  Is exceptionally large 
B)  Is an expression vector 
C)  Contains pieces of a viral genome 
D)  Contains a selectable marker 
E)  None of the answers are correct

Question 16

Which of the following is used to identify a specific protein in a library?

Accepted Answer

A)  Western blotting 
B)  Northern blotting 
C)  Southern blotting 
D)  Eastern blotting 
A)  Western blotting 
B)  Northern blotting 
C)  Southern blotting 
D)  Eastern blotting

Question 17

conditions are essential in a Southern blot experiment if the researcher wants to identify the closest DNA match to the probe.

Accepted Answer

A)  High stringency 
B)  Low stringency 
A)  High stringency 
B)  Low stringency

Question 18

A vector is a small segment of DNA that a gene of interest is inserted into for cloning.

Accepted Answer

A) True 
 B)False

Question 19

A cDNA library differs from a DNA library in which way?

Accepted Answer

A)  It consists solely of RNA molecules 
B)  It contains many copies of the gene of interest 
C)  It contains only coding sequences, not introns 
D)  It is typically 10-100 times the size of a DNA library 
A)  It consists solely of RNA molecules 
B)  It contains many copies of the gene of interest 
C)  It contains only coding sequences, not introns 
D)  It is typically 10-100 times the size of a DNA library

Question 20

Which of the following is needed for a PCR reaction?

Accepted Answer

A)  Template DNA 
B)  Primers 
C)  DNA polymerase 
D)  Deoxynucleotide triphosphates 
E)  All of the answers are correct 
A)  Template DNA 
B)  Primers 
C)  DNA polymerase 
D)  Deoxynucleotide triphosphates 
E)  All of the answers are correct

Exam 18: Recombinant Dna Technology

Which of the following is used to identify a specific RNA in a library?

Which of the following is NOT a stage of a PCR reaction?

What is the purpose of RNaseH in the making of cDNA?

Which of the following would contain both vector DNA and chromosomal DNA?

How might a probe for a colony hybridization experiment be obtained?

The use of fluorescent-labeled dideoxynucleotides is a characteristic of what type of sequencing?

Which of the following is correct regarding the first cloning experiment?

Which of the following techniques may be used to determine DNA-protein interactions?

Small circular pieces of bacterial DNA that are used as vectors in cloning experiments are called .

Antibodies can be used as a probe for which of the following techniques?

DNA ligase is needed in a cloning experiment for what reason?

Dideoxy nucleotides are used in which of the following techniques?

What occurs during the annealing stage of a PCR reaction?

The DNA Pol III enzyme can use RNA to make a DNA strand.

The presence of a specific plasmid in a bacterial cell may be determined if the plasmid .

Which of the following is used to identify a specific protein in a library?

conditions are essential in a Southern blot experiment if the researcher wants to identify the closest DNA match to the probe.

A vector is a small segment of DNA that a gene of interest is inserted into for cloning.

A cDNA library differs from a DNA library in which way?

Which of the following is needed for a PCR reaction?

Overview of Genetics

Mendelian Inheritance

Reproduction and Chromosome Transmission

Extensions of Mendelian Inheritance

Non-Mendelian Inheritance

Genetic Linkage and Mapping in Eukaryotes

Genetic Transfer and Mapping in Bacteria and

Variation in Chromosome Structure and Number

Molecular Structure of Dna and Rna

Chromosome Organization and Molecular Structure

Dna Replication

Gene Transcription and Rna Modification

Translation of Mrna

Gene Regulation in Bacteria and Bacteriophages

Gene Regulation in Eukaryotes

Gene Mutation and Dna Repair

Recombination and Transposition at the Molecular

Biotechnology

Genomics I: Analysis of Dna

Genomics II: Functional Genomics, Proteomics, and

Medical Genetics and Cancer

Developmental Genetics

Population Genetics

Quantitative Genetics

Evolutionary Genetics

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