Deck 18: Drug Metabolomics and Proteomics Analysis in Drug Delivery and Discovery

Question 1

What is proteomics?

A) Study of drugs in biological system
B) Study of peptides in biological system
C) Study of drug metabolism in biological system
D) Study of proteome and their fragments including peptides and free amino acids

Answer 1

D

Question 2

Where proteomics approaches are used?

A) Post-translational modifications studies
B) Identification of genomic information in cells
C) Organelle constituent identification
D) a and b
E) a and c
F) None of the above
G) d and e

Answer 2

G

Question 3

What is residue of protein?

A) Carbohydrate
B) glycogen
C) amino acid
D) aglycone

Answer 3

C

Question 4

Peptides are linear short polymers of amino acids with less than fifty residues?

Question 5

What different non-radioactive isotopic labeling techniques are used in protein purification and isolation for proteomic?

A) Tandem affinity purification (TAP)
B) Isotope-coded affinity tagging (ICAT)
C) Isobaric (iTRAQ) tag and
D) Stable isotope labeling by amino acid in cell culture (SILAC)
E) All of the above

Question 6

What is the use of stable isotope labeling by amino acid in cell culture (SILAC)?

A) Identification and quantification of proteins such as phosphorylation and methylation
B) Post translational modifications of proteins,DNA and RNA substrates occurs on arginine and lysine residues
C) All of the above
D) None of the above

Question 7

Covalent post-translational modifications occurs in a residue of proteome?

Question 8

Post translational modifications of proteins,DNA and RNA substrates occurs on arginine and lysine residues

Question 9

Phosphorylation is a chemical process in which a phosphate group is added to an organic molecules.

Question 10

Fill in the blanks: Phosphorylation is associated with respiration and photosynthesis which occurs in _________and _____________.

Question 11

Glycosylation is used to study multiple functions in the cell for molecular targeting and cell-cell recognition particularly with ________,__________,and ___________.

Question 12

Phosphorylation is a metabolic & signaling processes particularly take place in which amino acid residues____________,____________,and __________ .

Question 13

Hydroxylation reaction is used to increase H-bonding & glycosylation sites which is primarily occurs on Pro,Lys.

Question 14

Hydroxylation reaction causes oxidative degradation of organic compounds in air and it is not extremely important in detoxification for rapid renal excretion.

Question 15

In which chemical process,lipophilic compounds converts into water-soluble compounds for rapid renal excretion to reduce toxicity?
A) None of the above

A) Post translational modifications
B) Hydroxylation
C) Phosphorylation
D) Glycosylation

Question 16

Acetylation chemical process used for charge weaken interactions with DNA and which is primarily takes place in unique amino acid residue. What is that?

A) Lucien
B) Glycine
C) Lysine
D) Threonine
E) All of the above

Question 17

In which chemical process co-translational and post-translational modification of proteins occurs in the cell?

A) Hydroxylation
B) Phosphorylation
C) Acetylation
D) None of the above

Question 18

What type of chemical groups are introduced by acetylation reaction in the cell?

A) Acetyl group
B) Acetoxy group
C) Both of these answers are correct
D) None of the above

Question 19

What kind of amino acid residue play an important role in methylation?

A) Valine
B) Glycin
C) Lysine
D) All of the above

Question 20

By which enzyme catalysis,the carboxylation reaction introduces carbon dioxide in the DNA?

A) Cytochrome-P450
B) β-glycosidase (Emulsin )
C) Ribulose 1,5-bisphosphate carboxylase (Rubisco).
D) None of the above

Question 21

Carboxylation reaction takes place in a posttranslational modification of glutamate residues to anionic γ-carboxyglutamate which can bind cationic molecules

Question 22

What is the name of reaction which takes place in primary amide groups and secondary and tertiary amine groups?

A) Transamination
B) Transamidation
C) Both of the above
D) None of the above

Question 23

Transamidation reaction is primarily occurs in----

A) Alanine and Arginine
B) Valine and Glycine
C) Glutamine and Lysine
D) None of the above

Question 24

What is the main reason for use of mass spectrometry techniques in protein and peptide analysis in drug delivery?

A) Pharmacokinetic (PK-ADME)
B) Pharmacodynamics (PD)
C) a and b
D) None of the above

Question 25

What is the full form for LC-MS/MS?

A) High Performance Liquid Chromatography (HPLC)
B) Ultra Performance Liquid Chromatography(UPLC)
C) Ultra Fast Liquid Chromatography(UFLC)
D) Gas liquid chromatography (GC-MS)
E) Liquid Chromatography tandem mass spectrometry(LC-tandem MS)

Question 26

What type of tandem mass spectrometry ionization techniques have been used for proteomics analysis?

A) Matrix Assisted Laser Desorption Ionization (MALDI)
B) Electrospray Ionization (ESI)
C) Atmospheric Pressure Chemical Ionization (APCI)
D) Fast Atom Bombardment (FAB) ionization
E) a and b

Question 27

Where electrospray Ionization (ESI) is occurs in the tandem mass spectrometry?

A) Before entering the analyzer
B) After entering the analyzer
C) Both of the above
D) None of the above

Question 28

What types of mass analyzers used in protein and peptide analysis?

A) Quadrupole analyzers,
B) Quadrupole ion trap analyzers,
C) Time-of-flight analyzers,
D) Ion cyclotron resonance and
E) Fourier transform analyzers Before entering the analyzer
F) All of the above
G) None of the above

Question 29

What are the most common available isotopic molecular weights for carbon (C)?

A) Carbon (C-12): 12.000000Da
B) Carbon (C-13): 13.003355Da
C) a and b
D) None of the above

Question 30

What are the most common available isotopic molecular weights for nitrogen (N)?

A) Nitrogen (N-15): 15.000108Da
B) Nitrogen (N-14): 14.003074Da
C) a and b
D) None of the above

Question 31

What are the most common available isotopic molecular weights for oxygen (O)?

A) Oxygen (O-17): 16.999133Da
B) Oxygen (O-18): 17.999169Da
C) Oxygen (O-16): 15.994915Da
D) a ,b and c
E) None of the above

Question 32

What are the most common available isotopic molecular weights for sulfur (S)?

A) Sulfur (S-32): 31.972970Da
B) Sulfur (S-33): 32.971456Da
C) Sulfur (S-34): 33.967866Da
D) Sulfur (S-35): 35.967080Da
E) None of the above

Question 33

What are the most common available isotopic molecular weights for hydrogen (H)?

A) Hydrogen (H-2): 2.014Da
B) Hydrogen (H-1): 1.007825Da
C) Hydrogen (H-1): 1.407825Da
D) None of the above

Question 34

What is "Omics Science"?

A) Biological research ending omics
B) Biotransformation of compounds
C) a and b
D) None of the above

Question 35

Genomics,lipidomics,metabolomics,metabonomics,pharmacogenomics,pharmacomicrobiomics,proteomics,psychogenomics,toxicogenomics and nutrigenomics are called as

A) Biological Science
B) Omics Sciences
C) a and b
D) None of the above

Question 36

Metabonomics is defined as

A) Biotransformation of small-molecules in biological fluids using analytical techniques such as LC-MS/MS or NMR
B) Study of genes that influence phenotype response to drugs
C) a and b
D) None of the above

Question 37

What type of detectors used in mass spectrometry?

A) Photon multiplier detector
B) Electron multiplier detector
C) a and b
D) None of the above

Question 38

What is the most common technique used for protein digestion?

A) Liquid -liquid extraction
B) Solid phase extraction
C) Trypsin digestion
D) Protein precipitation

Question 39

What type of database search programs are used in proteomics analysis?
a) Mascot, http://www.matrixscience.com/
b) Masslynx http://www.waters.com
c) MS-Tag/MS-Seq http://prospector.ucsf.edu/
d) PeptideSearch http://www.narrador.embl-heidelberg.de/GroupPages/Homepage.html
e) PepFrag http://prowl.rockefeller.edu/PROWL
f) All of the above
g) None of the above

Question 40

What is the description of bottom-up proteomics?

A) Proteins and its fragments analysis
B) Peptides and amino acid sequence analysis
C) None of the above
D) All of the above

Question 41

Protonated molecular ions [M+H]⁺ are formed in

A) Negative ionization mode in mass spectrometry ionization
B) Positive ionization mode in mass spectrometry ionization
C) None of the above
D) Both ionization modes

Question 42

What is mass spectrometry (MS) ?

A) Analytical technique use in separation of compounds
B) Analytical technique use in purification of compounds
C) Analytical tool used for measuring the molecular mass of a sample
D) None of the above

Question 43

Large biomolecules,molecular masses can be measured to within an accuracy of _____ of the total molecular mass?

Question 44

How can mass spectrometry help in proteomics except?

A) Accurate molecular weight measurements
B) Amino acid sequencing
C) Protein structure
D) Reaction monitoring

Question 45

Mass spectrometers can be divided into three fundamental parts,namely the ___,___,and ___.

Question 46

Ions are extracted into the analyser region of the mass spectrometer according to their mass (m)-to-charge (z) ratios (m/z)

Question 47

In positive ionization mode tandem (MS-MS),a trace of ___ is usually added to induce ___ of the sample molecules

Question 48

Proteins and peptides are generally analyzed in ___ conditions and saccharides and oligonucleotides in ___.

Question 49

Samples (M),multiply charged molecular-related ions such as ___ in positive ionisation mode and ___ in negative ionisation mode.

Question 50

MALDI method predominantly generate a singly charged molecular-related ions regardless of the molecular mass.

Question 51

Match the ABC transporter with the respective number of TMDs.
MRP2

A) 7
B) 13
C) 18

Question 52

Match the ABC transporter with the respective number of TMDs.
BCRP

A) 8
B) 14
C) 19

Question 53

Match the routes of drug administration with their respective areas of action.
Brain

A) Intra-articular
B) Intra-thecal
C) Intra-peritoneal
D) Intra-medullary

Question 54

Match the routes of drug administration with their respective areas of action.
Bone marrow

A) Intra-articular
B) Intra-thecal
C) Intra-peritoneal
D) Intra-medullary

Question 55

Match the routes of drug administration with their respective areas of action.
Joints

A) Intra-articular
B) Intra-thecal
C) Intra-peritoneal
D) Intra-medullary