Deck 1: Histology Itsmethods of Study

Question 1

In preparing tissue for routine light microscopic study, which procedure immediately precedes clearing the specimen with an organic solvent?

A) Dehydration
B) Fixation
C) Staining
D) Clearing
E) Embedding

Answer 1

Dehydration

Question 2

Which of the following staining procedures relies on the cationic and anionic properties of the material to be stained?

A) Enzyme histochemistry
B) PAS reaction
C) H & E staining
D) Immunohistochemistry
E) Metal impregnation techniques

Answer 2

H & E staining

Question 3

In a light microscope used for histology, resolution and magnification of cells are largely dependent on which component?

A) Condenser
B) Objective lens
C) Eyepieces or ocular lenses
D) Specimen slide
E) The control for illumination intensity

Answer 3

Objective lens

Question 4

Cellular storage deposits of glycogen, a free polysaccharide, could best be detected histologically using what procedure?

A) Autoradiography
B) Electron microscopy
C) Enzyme histochemistry
D) H&E staining
E) PAS reaction

Question 5

Adding heavy metal compounds to the fixative and ultrathin sectioning of the embedded tissue with a glass knife are techniques used for which histologic procedure?

A) Scanning electron microscopy
B) Fluorescent microscopy
C) Enzyme histochemistry
D) Confocal microscopy
E) TEM

Question 6

Resolution in electron microscopy greatly exceeds that of light microscopy due to which of the following?

A) The wavelength of the electrons in the microscope beam is shorter than that of a beam of light.
B) The lenses of an electron microscope are of greatly improved quality.
C) For electron microscopy the tissue specimen does not require staining.
D) The electron microscope allows much greater magnification of a projected image than a light microscope provides.
E) An electron microscope can be much more finely controlled than a light microscope.

Question 7

Microscopic autoradiography uses radioactivity and can be employed to study what features in a tissue section?

A) The types of enzymes found in various cell locations
B) Cellular sites where various macromolecules are synthesized
C) The sequences of mRNA made in the cells
D) The dimensions of structures within the cells
E) The locations of genes transcribed for specific mRNA

Question 8

To identify and localize a specific protein within cells or the ECM, one would best use what approach?

A) Autoradiography
B) Enzyme histochemistry
C) Immunohistochemistry
D) TEM
E) Polarizing microscopy

Question 9

In situ hybridization is a histologic technique used to visualize what type of macromolecule?

A) Proteins
B) Carbohydrates
C) Certain enzymes
D) Nucleic acids
E) Lipids

Question 10

Hospital laboratories frequently use unfixed, frozen tissue specimens sectioned with a cryostat for rapid staining, microscopic examination, and diagnosis of pathologic conditions. Besides saving much time by avoiding fixation and procedures required for paraffin embedding, frozen sections retain and allow study of what macromolecules normally lost in the paraffin procedure?

A) Carbohydrates
B) Small mRNA
C) Basic proteins
D) Acidic proteins
E) Lipids