Exam 1: Histology Itsmethods of Study

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In preparing tissue for routine light microscopic study, which procedure immediately precedes clearing the specimen with an organic solvent?

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A

Which of the following staining procedures relies on the cationic and anionic properties of the material to be stained?

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C

Adding heavy metal compounds to the fixative and ultrathin sectioning of the embedded tissue with a glass knife are techniques used for which histologic procedure?

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E

Microscopic autoradiography uses radioactivity and can be employed to study what features in a tissue section?

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To identify and localize a specific protein within cells or the ECM, one would best use what approach?

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Resolution in electron microscopy greatly exceeds that of light microscopy due to which of the following?

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Cellular storage deposits of glycogen, a free polysaccharide, could best be detected histologically using what procedure?

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Hospital laboratories frequently use unfixed, frozen tissue specimens sectioned with a cryostat for rapid staining, microscopic examination, and diagnosis of pathologic conditions. Besides saving much time by avoiding fixation and procedures required for paraffin embedding, frozen sections retain and allow study of what macromolecules normally lost in the paraffin procedure?

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In a light microscope used for histology, resolution and magnification of cells are largely dependent on which component?

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In situ hybridization is a histologic technique used to visualize what type of macromolecule?

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