Deck 19: Analysis of Gene Expression

Question 1

Primer extension is used to .

A)determine the transcriptional start site
B)locate the binding of RNA polymerase
C)determine the interaction of RNA and proteins
D)generate primers for PCR

Answer 1

A

Question 2

Reporter systems are most useful for .

A)monitoring gene expression
B)determining DNA-protein interactions
C)identifying the product of biochemical reactions
D)constructing large numbers of clones in bacteria

Answer 2

A

Question 3

Which of the following techniques would not be useful for determining the number of mRNA copies present in a cell?

A)SAGE
B)DNA microarrays
C)Quantitative PCR
D)Primer extension

Answer 3

D

Question 4

Microarrays and SAGE have the ability to monitor .

A)large numbers of mRNAs simultaneously
B)small numbers of specific mRNAs
C)proteins expressed under a given condition
D)the binding of a protein to a DNA or RNA molecule

Question 5

Deletion analysis allows a researcher to examine the effects of regulatory proteins on gene expression.

Question 6

A specific mRNA may be detected using hybridization.

A)Southern
B)Western
C)Northern
D)Southwestern

Question 7

DNase I protection assays or DNA footprinting is useful for .

A)determining the start of transcription
B)determining the location of a DNA binding sequence
C)preventing contamination of RNA in primer extension samples
D)none of the above

Question 8

Levels of transcription for a specific gene are measured using all of the following except .

A)quantitative PCR
B)reporter genes
C)primer extension
D)DNA microarrays

Question 9

Where might green fluorescent protein be found?

A)fireflies
B)jellyfish
C)bacteria
D)algae

Question 10

DNA microarrays are appropriate for investigating the expression of one or a few genes.

Question 11

How might the potential binding of a protein to a specific fragment of DNA be investigated?

A)primer extension
B)western blotting
C)gel mobility shift assays
D)isoelectric focusing