Question 1

DNase I protection assays or DNA footprinting is useful forـــــــــــــــــــــــ .

Accepted Answer

A) determining the start of transcription 
B) determining the location of a DNA binding sequence 
C) preventing contamination of RNA in primer extension samples 
D) none of the above 
A) determining the start of transcription 
B) determining the location of a DNA binding sequence 
C) preventing contamination of RNA in primer extension samples 
D) none of the above B

Question 2

Primer extension is used toــــــــــــــــــــــ .

Accepted Answer

A) determine the transcriptional start site 
B) locate the binding of RNA polymerase 
C) determine the interaction of RNA and proteins 
D) generate primers for PCR 
A) determine the transcriptional start site 
B) locate the binding of RNA polymerase 
C) determine the interaction of RNA and proteins 
D) generate primers for PCR A

Question 3

DNA microarrays are appropriate for investigating the expression of one or a few genes.

Accepted Answer

A) True 
 B)False  False

Question 4

Deletion analysis allows a researcher to examine the effects of regulatory proteins on gene expression.

Accepted Answer

A) True 
 B)False

Question 5

A specific mRNA may be detected using ـــــــــــــــــــhybridization.

Accepted Answer

A) Southern 
B) Western 
C) Northern 
D) Southwestern 
A) Southern 
B) Western 
C) Northern 
D) Southwestern

Question 6

Microarrays and SAGE have the ability to monitorــــــــــــــــــــ .

Accepted Answer

A) large numbers of mRNAs simultaneously 
B) small numbers of specific mRNAs 
C) proteins expressed under a given condition 
D) the binding of a protein to a DNA or RNA molecule 
A) large numbers of mRNAs simultaneously 
B) small numbers of specific mRNAs 
C) proteins expressed under a given condition 
D) the binding of a protein to a DNA or RNA molecule

Question 7

Reporter systems are most useful for ـــــــــــــــــــــ.

Accepted Answer

A) monitoring gene expression 
B) determining DNA-protein interactions 
C) identifying the product of biochemical reactions 
D) constructing large numbers of clones in bacteria 
A) monitoring gene expression 
B) determining DNA-protein interactions 
C) identifying the product of biochemical reactions 
D) constructing large numbers of clones in bacteria

Question 8

Which of the following techniques would not be useful for determining the number of mRNA copies present in a cell?

Accepted Answer

A) SAGE 
B) DNA microarrays 
C) Quantitative PCR 
D) Primer extension 
A) SAGE 
B) DNA microarrays 
C) Quantitative PCR 
D) Primer extension

Question 9

Where might green fluorescent protein be found?

Accepted Answer

A) fireflies 
B) jellyfish 
C) bacteria 
D) algae 
A) fireflies 
B) jellyfish 
C) bacteria 
D) algae

Question 10

How might the potential binding of a protein to a specific fragment of DNA be investigated?

Accepted Answer

A) primer extension 
B) western blotting 
C) gel mobility shift assays 
D) isoelectric focusing 
A) primer extension 
B) western blotting 
C) gel mobility shift assays 
D) isoelectric focusing

Question 11

Levels of transcription for a specific gene are measured using all of the following exceptـــــــــــــــــــ .

Accepted Answer

A) quantitative PCR 
B) reporter genes 
C) primer extension 
D) DNA microarrays 
A) quantitative PCR 
B) reporter genes 
C) primer extension 
D) DNA microarrays

DNase I protection assays or DNA footprinting is useful forـــــــــــــــــــــــ .

Primer extension is used toــــــــــــــــــــــ .

DNA microarrays are appropriate for investigating the expression of one or a few genes.

Deletion analysis allows a researcher to examine the effects of regulatory proteins on gene expression.

A specific mRNA may be detected using ـــــــــــــــــــhybridization.

Microarrays and SAGE have the ability to monitorــــــــــــــــــــ .

Reporter systems are most useful for ـــــــــــــــــــــ.

Which of the following techniques would not be useful for determining the number of mRNA copies present in a cell?

Where might green fluorescent protein be found?

How might the potential binding of a protein to a specific fragment of DNA be investigated?

Levels of transcription for a specific gene are measured using all of the following exceptـــــــــــــــــــ .

Cells and Organisms

Basic Genetics

DNA, RNA, and Protein

Genes, genomes, and DNA

Manipulation of Nucleic Acids

Polymerase Chain Reaction

Recombinant DNA Technology

DNA Sequencing

Genomics Systems Biology

Cell Division and DNA Replication

Transcription of Genes

Processing of RNA

Protein Synthesis

Protein Structure and Function

Proteomics

Regulation of Transcription in Prokaryotes

Regulation of Transcription in Eukaryotes

Regulation at the RNA Level

Plasmids

Viruses

Mobile DNA

Mutations and Repair

Recombination

Bacterial Genetics

Molecular Evolution

Filters

Exam 19: Analysis of Gene Expression

DNase I protection assays or DNA footprinting is useful forـــــــــــــــــــــــ .

Primer extension is used toــــــــــــــــــــــ .

DNA microarrays are appropriate for investigating the expression of one or a few genes.

Deletion analysis allows a researcher to examine the effects of regulatory proteins on gene expression.

A specific mRNA may be detected using ـــــــــــــــــــhybridization.

Microarrays and SAGE have the ability to monitorــــــــــــــــــــ .

Reporter systems are most useful for ـــــــــــــــــــــ.

Which of the following techniques would not be useful for determining the number of mRNA copies present in a cell?

Where might green fluorescent protein be found?

How might the potential binding of a protein to a specific fragment of DNA be investigated?

Levels of transcription for a specific gene are measured using all of the following exceptـــــــــــــــــــ .

Cells and Organisms

Basic Genetics

DNA, RNA, and Protein

Genes, genomes, and DNA

Manipulation of Nucleic Acids

Polymerase Chain Reaction

Recombinant DNA Technology

DNA Sequencing

Genomics Systems Biology

Cell Division and DNA Replication

Transcription of Genes

Processing of RNA

Protein Synthesis

Protein Structure and Function

Proteomics

Regulation of Transcription in Prokaryotes

Regulation of Transcription in Eukaryotes

Regulation at the RNA Level

Plasmids

Viruses

Mobile DNA

Mutations and Repair

Recombination

Bacterial Genetics

Molecular Evolution

Filters