Deck 11: General Transcription Factors in Eukaryotes

Question 1

One approach to studying the phosphoryation of the CTD of RNA polymerase II would be

A)the use of photo-crosslinking.
B)the use of [ $\alpha$ -32]ATP in the presence of a phosphorylase.
C)the use of [ $\gamma$ -32]ATP in the presence of a kinase.
D)the use of photo-crosslinking and the use of [ $\gamma$ -32]ATP in the presence of a kinase.
E)the use of photo-crosslinking and the use of [ $\alpha$ -32]ATP in the presence of a phosphorylase.

Answer 1

the use of [ $\gamma$ -32]ATP in the presence of a kinase.

Question 2

Which of the following is a possible effect of creating a mutant cell line with reduced levels of TAF1?

A)Initiation complex would be slow to form.
B)Polymerase II will show weak binding to the promoter.
C)Proofreading activity will be impaired.
D)The DAB complex will dissociate.
E)None of the choices are correct.

Answer 2

C

Question 3

Which of the following would you expect to bind to a chromatography column made of a resin conjugated to UBF?

A)Sp1
B)SL1
C)TFIIB
D)TFIIH
E)TFIIE

Answer 3

B

Question 4

Which of the following are true about the role of the core TAFs?

A)They interact with core promoter elements.
B)They interact with activators.
C)They are evolutionarily conserved.
D)They interact with core promoter elements and they interact with activators are correct.
E)They interact with core promoter elements,they interact with activators,and they are evolutionarily conserved are correct.

Question 5

Determine which of the following would most likely lead to a dramatic reduction in transcription.

A)absence of the TATA box
B)removal of some TAFs
C)removal of TAF1
D)absence of the TATA box and removal of some TAFs
E)none of the choices are correct.

Question 6

TFIID collaborates with TFIIA to bind to the TATA box.

Question 7

Which of the following are required for class II gene transcription?

A)TFIIB
B)TFIIIC
C)TFIID
D)TFIIB and TFIIIC
E)TFIIIC and TFIID

Question 8

Which of the following is not a part of TFIID?

A)-45 region
B)TBP
C)TBP-associated factors
D)-45 region and TBP
E)TBP and TBP-associated factors

Question 9

Human SL1 is composed of all of the following except

A)TBP.
B)TAF_I30.
C)TAF_I 110.
D)TAF_I63.
E)TAF_I48.

Question 10

Which of the following is considered a universal transcription factor?

A)TFIIA
B)TFIIB
C)TFIID
D)TFIIF
E)TFIIH

Question 11

Predict the consequence of blocking the action of TFIIF.

A)The DAB complex will not form.
B)TFIIA will not bind to TFIID.
C)Polymerase may not bind to the DAB complex.
D)The DAB complex will not form and TFIIA will not bind to TFIID are correct.
E)TFIIA will not bind to TFIID and polymerase may not bind to the DAB complex are correct.

Question 12

The archaea,P.woesei,produces a transcription factor that is similar to which of the following in eukaryotes?

A)TFIIA
B)TFIIB
C)TFIID
D)TFIIF
E)TFIIH

Question 13

Tijan's experiment to study the mechanism of TAF binding involved which of the following?

A)use of E1B and E4 promoter regions
B)in vitro transcription assay
C)DNAse I footprinting
D)photo-crosslinking
E)all of the choices are correct.

Question 14

The following are features of TAF1:

A)contains DNase I domain.
B)is a histone acetyltransferase.
C)is a protein kinase.
D)contains DNase I domain and is a histone acetyltransferase.
E)is a histone acetyltransferase and is a protein kinase.

Question 15

____________________ is also known as the preinitiation complex.

Question 16

Which of the following is the correct order of addition of general transcription factors to the initiation complex?

A)TFIIA+TFIID,TFIIE,TFIIIIB,TFIIF + pol II,TFIIH
B)TFIIA+TFIID,TFIIB,TFIIH,TFIIF + pol II,TFIIE
C)TFIIA+TFIID,TFIIB,TFIIF + pol II,TFIIE,TFIIH
D)TFIIA+TFIID,TFIIH,TFIIIIB,TFIIF + pol II,TFIIE
E)TFIIF + pol II,TFIIA+TFIID,TFIIH,TFIIIIB,TFIIE

Question 17

What is the effect on the activity of RNA polymerase I if TAF1 is deleted from the promoter?

A)Polymerase I will not form the preinitiation complex.
B)Phosphorylation of the DNA-binding domain will be impaired.
C)Elongation will be slowed.
D)Polymerase I will not form the preinitiation complex and phosphorylation of the DNA-binding domain will be impaired are correct.
E)None of the choices is correct.

Question 18

You are planning an experiment to look at the specific binding of the TBP to the promoter.Which of the following would be a suitable negative control for this experiment?

A)a DNA sequence made with multiple cytosines
B)a DNA strand containing the TATA box linked to multiple adenine
C)a DNA strand containing the TATA box linked to multiple guanines
D)a DNA sequence made with multiple cytosines and a DNA strand containing the TATA box linked to multiple adenine
E)a DNA sequence made with multiple cytosines,a DNA strand containing the TATA box linked to multiple adenine,a DNA strand containing the TATA box linked to multiple guanines

Question 19

Select a likely effect of reducing the level of TIF-IB from a yeast cell line.

A)Polymerase will not recognize the promoter.
B)The level of transcription initiation from class I promoters will be low.
C)Polymerase will not associate with DAB.
D)Polymerase will not recognize the promoter and the level of transcription initiation from class I promoters will be low are correct.
E)The level of transcription initiation from class I promoters will be low and polymerase will not associate with DAB are correct.

Question 20

General transcription factors combine with RNA polymerase to form a ______ ___________.

Question 21

The DNA-binding domain of TBP is located in the carboxyl-terminal domain.

Question 22

RNA polymerase prevents the binding of TFIIF to the promoter region.

Question 23

TAFs are universally required for the transcription of class II genes.

Question 24

A promoter region lacking the TATA box will not bind to TFIID.

Question 25

The carboxyl-terminal domain of polymerase is phosphorylated prior to the formation of the initiation complex.

Question 26

Based on experimental evidence,RNA polymerase II binds to a region extending from -64 to +74.

Question 27

The TBP portion of the general transcription factor TFIID binds only to the major groove of the TATA box.

Question 28

At least eight TAFs are associated with TFIID.

Question 29

Classical class III genes require two factors,TFIIIB and C,in order to form a preinitiation complex with the polymerase.