Question 1

RNA polymerase prevents the binding of TFIIF to the promoter region.

Accepted Answer

A) True 
 B)False  False

Question 2

Based on experimental evidence,RNA polymerase II binds to a region extending from -64 to +74.

Accepted Answer

A) True 
 B)False  False

Question 3

Determine which of the following would most likely lead to a dramatic reduction in transcription.

Accepted Answer

A) absence of the TATA box 
B) removal of some TAFs 
C) removal of TAF1 
D) absence of the TATA box and removal of some TAFs 
E) none of the choices are correct. 
A) absence of the TATA box 
B) removal of some TAFs 
C) removal of TAF1 
D) absence of the TATA box and removal of some TAFs 
E) none of the choices are correct. E

Question 4

One approach to studying the phosphoryation of the CTD of RNA polymerase II would be

Accepted Answer

A) the use of photo-crosslinking. 
B) the use of [$\alpha$-32]ATP in the presence of a phosphorylase. 
C) the use of [$\gamma$-32]ATP in the presence of a kinase. 
D) the use of photo-crosslinking and the use of [$\gamma$-32]ATP in the presence of a kinase. 
E) the use of photo-crosslinking and the use of [$\alpha$-32]ATP in the presence of a phosphorylase. 
A) the use of photo-crosslinking. 
B) the use of [$\alpha$-32]ATP in the presence of a phosphorylase. 
C) the use of [$\gamma$-32]ATP in the presence of a kinase. 
D) the use of photo-crosslinking and the use of [$\gamma$-32]ATP in the presence of a kinase. 
E) the use of photo-crosslinking and the use of [$\alpha$-32]ATP in the presence of a phosphorylase.

Question 5

TAFs are universally required for the transcription of class II genes.

Accepted Answer

A) True 
 B)False

Question 6

____________________ is also known as the preinitiation complex.

Accepted Answer

The answer of ____________________ is also known as the preinitiation...

Question 7

Which of the following are true about the role of the core TAFs?

Accepted Answer

A) They interact with core promoter elements. 
B) They interact with activators. 
C) They are evolutionarily conserved. 
D) They interact with core promoter elements and they interact with activators are correct. 
E) They interact with core promoter elements,they interact with activators,and they are evolutionarily conserved are correct. 
A) They interact with core promoter elements. 
B) They interact with activators. 
C) They are evolutionarily conserved. 
D) They interact with core promoter elements and they interact with activators are correct. 
E) They interact with core promoter elements,they interact with activators,and they are evolutionarily conserved are correct.

Question 8

The TBP portion of the general transcription factor TFIID binds only to the major groove of the TATA box.

Accepted Answer

A) True 
 B)False

Question 9

The archaea,P.woesei,produces a transcription factor that is similar to which of the following in eukaryotes?

Accepted Answer

A) TFIIA 
B) TFIIB 
C) TFIID 
D) TFIIF 
E) TFIIH 
A) TFIIA 
B) TFIIB 
C) TFIID 
D) TFIIF 
E) TFIIH

Question 10

Tijan's experiment to study the mechanism of TAF binding involved which of the following?

Accepted Answer

A) use of E1B and E4 promoter regions 
B) in vitro transcription assay 
C) DNAse I footprinting 
D) photo-crosslinking 
E) all of the choices are correct. 
A) use of E1B and E4 promoter regions 
B) in vitro transcription assay 
C) DNAse I footprinting 
D) photo-crosslinking 
E) all of the choices are correct.

Question 11

General transcription factors combine with RNA polymerase to form a ______ ___________.

Accepted Answer

preinitiat

Question 12

What is the effect on the activity of RNA polymerase I if TAF1 is deleted from the promoter?

Accepted Answer

A) Polymerase I will not form the preinitiation complex. 
B) Phosphorylation of the DNA-binding domain will be impaired. 
C) Elongation will be slowed. 
D) Polymerase I will not form the preinitiation complex and phosphorylation of the DNA-binding domain will be impaired are correct. 
E) None of the choices is correct. 
A) Polymerase I will not form the preinitiation complex. 
B) Phosphorylation of the DNA-binding domain will be impaired. 
C) Elongation will be slowed. 
D) Polymerase I will not form the preinitiation complex and phosphorylation of the DNA-binding domain will be impaired are correct. 
E) None of the choices is correct.

Question 13

Which of the following is the correct order of addition of general transcription factors to the initiation complex?

Accepted Answer

A) TFIIA+TFIID,TFIIE,TFIIIIB,TFIIF + pol II,TFIIH 
B) TFIIA+TFIID,TFIIB,TFIIH,TFIIF + pol II,TFIIE 
C) TFIIA+TFIID,TFIIB,TFIIF + pol II,TFIIE,TFIIH 
D) TFIIA+TFIID,TFIIH,TFIIIIB,TFIIF + pol II,TFIIE 
E) TFIIF + pol II,TFIIA+TFIID,TFIIH,TFIIIIB,TFIIE 
A) TFIIA+TFIID,TFIIE,TFIIIIB,TFIIF + pol II,TFIIH 
B) TFIIA+TFIID,TFIIB,TFIIH,TFIIF + pol II,TFIIE 
C) TFIIA+TFIID,TFIIB,TFIIF + pol II,TFIIE,TFIIH 
D) TFIIA+TFIID,TFIIH,TFIIIIB,TFIIF + pol II,TFIIE 
E) TFIIF + pol II,TFIIA+TFIID,TFIIH,TFIIIIB,TFIIE

Question 14

A promoter region lacking the TATA box will not bind to TFIID.

Accepted Answer

A) True 
 B)False

Question 15

Which of the following are required for class II gene transcription?

Accepted Answer

A) TFIIB 
B) TFIIIC 
C) TFIID 
D) TFIIB and TFIIIC 
E) TFIIIC and TFIID 
A) TFIIB 
B) TFIIIC 
C) TFIID 
D) TFIIB and TFIIIC 
E) TFIIIC and TFIID

Question 16

The DNA-binding domain of TBP is located in the carboxyl-terminal domain.

Accepted Answer

A) True 
 B)False

Question 17

Which of the following is considered a universal transcription factor?

Accepted Answer

A) TFIIA 
B) TFIIB 
C) TFIID 
D) TFIIF 
E) TFIIH 
A) TFIIA 
B) TFIIB 
C) TFIID 
D) TFIIF 
E) TFIIH

Question 18

You are planning an experiment to look at the specific binding of the TBP to the promoter.Which of the following would be a suitable negative control for this experiment?

Accepted Answer

A) a DNA sequence made with multiple cytosines 
B) a DNA strand containing the TATA box linked to multiple adenine 
C) a DNA strand containing the TATA box linked to multiple guanines 
D) a DNA sequence made with multiple cytosines and a DNA strand containing the TATA box linked to multiple adenine 
E) a DNA sequence made with multiple cytosines,a DNA strand containing the TATA box linked to multiple adenine,a DNA strand containing the TATA box linked to multiple guanines 
A) a DNA sequence made with multiple cytosines 
B) a DNA strand containing the TATA box linked to multiple adenine 
C) a DNA strand containing the TATA box linked to multiple guanines 
D) a DNA sequence made with multiple cytosines and a DNA strand containing the TATA box linked to multiple adenine 
E) a DNA sequence made with multiple cytosines,a DNA strand containing the TATA box linked to multiple adenine,a DNA strand containing the TATA box linked to multiple guanines

Question 19

Classical class III genes require two factors,TFIIIB and C,in order to form a preinitiation complex with the polymerase.

Accepted Answer

A) True 
 B)False

Question 20

At least eight TAFs are associated with TFIID.

Accepted Answer

A) True 
 B)False

Exam 11: General Transcription Factors in Eukaryotes

RNA polymerase prevents the binding of TFIIF to the promoter region.

Based on experimental evidence,RNA polymerase II binds to a region extending from -64 to +74.

Determine which of the following would most likely lead to a dramatic reduction in transcription.

One approach to studying the phosphoryation of the CTD of RNA polymerase II would be

TAFs are universally required for the transcription of class II genes.

____________________ is also known as the preinitiation complex.

Which of the following are true about the role of the core TAFs?

The TBP portion of the general transcription factor TFIID binds only to the major groove of the TATA box.

The archaea,P.woesei,produces a transcription factor that is similar to which of the following in eukaryotes?

Tijan's experiment to study the mechanism of TAF binding involved which of the following?

General transcription factors combine with RNA polymerase to form a ______ ___________.

What is the effect on the activity of RNA polymerase I if TAF1 is deleted from the promoter?

Which of the following is the correct order of addition of general transcription factors to the initiation complex?

A promoter region lacking the TATA box will not bind to TFIID.

Which of the following are required for class II gene transcription?

The DNA-binding domain of TBP is located in the carboxyl-terminal domain.

Which of the following is considered a universal transcription factor?

You are planning an experiment to look at the specific binding of the TBP to the promoter.Which of the following would be a suitable negative control for this experiment?

Classical class III genes require two factors,TFIIIB and C,in order to form a preinitiation complex with the polymerase.

At least eight TAFs are associated with TFIID.

A Brief History

The Molecular Nature of Genes

An Introduction to Gene Function

Molecular Cloning Methods

Molecular Tools for Studying Genes and Gene Activity

The Mechanism of Transcription in Bacteria

Operons: Fine Control of Bacterial Transcription

Major Shifts in Bacterial Transcription

Dna-Protein Interactions in Bacteria

Eukaryotic Rna Polymerases and Their Promoters

Transcription Activators in Eukaryotes

Chromatin Structure and Its Effect on Transcription

Rna Processing I: Splicing

Rna Processing II: Capping and Polyadenylation

Rna Processing: Other Post-Transcriptional Events

The Mechanism of Translation I: Initiation

The Mechanism of Translation II: Elongation and Termination

Ribosomes and Transfer Rna

Dna Replication,damage and Repair

Dna Replication II: Detailed Mechanism

Homologous Recombination

Transposition

Introduction to Genomics: Dna Sequencing on a Genomic Scale

Genomics II: Functional Genomics,proteomics and Bioinformatics

Filters

General transcription factors combine with RNA polymerase to form a _____.