Deck 10: Eukaryotic Rna Polymerases and Their Promoters

Question 1

Which of the following is true about Rpb4/7?

A)It binds to Rpb3 to facilitate melting of the DNA promoter.
B)It extends the dock region of polymerase,making it easier for transcription factors to bind.
C)It binds to DNA and facilitate elongation.
D)It binds to Rpb3 to facilitate melting of the DNA promoter and it extends the dock region of polymerase,making it easier for transcription factors to bind are correct.
E)It extends the dock region of polymerase,making it easier for transcription factors to bind and it binds to DNA and facilitate elongation are correct.

Answer 1

B

Question 2

You were asked to choose a method to separate a mixture of eukaryotic polymerases.Which of the following methods would you choose?

A)chromatography
B)western blotting
C)S1 nuclease assay
D)DNase 1 footprinting
E)chromatography and S1 nuclease assay

Answer 2

A

Question 3

Which of the following is ideal to study the function of the subunits of polymerase II?

A)Break the enzymes into component parts and gradually reassemble them.
B)Use mutational analysis.
C)Conduct DNA binding assays.
D)Break the enzymes into component parts and gradually reassemble them and use mutational analysis are correct.
E)Use mutational analysis and conduct DNA binding assays are correct.

Answer 3

B

Question 4

Which of the following is not a part of the core class II promoter?

A)TATA box
B)upstream element
C)BRE
D)DPE
E)Inr

Question 5

Which of the following is involved in the stress response?

A)Rpb2
B)Rpb3
C)Rpb4
D)Rpd8
E)Rpd10

Question 6

Roeder and Rutter showed that there are four polymerases operating in the eukaryotic cell.

Question 7

An experiment is planned to study the effect of reducing the transcription of the myosin gene.Which of the following inhibitors would be useful in this study?

A) $\alpha$ -amanitin
B)DMS
C)heparin
D)ammonium sulfate
E)none of the choices are correct.

Question 8

Which of the following subunits,when combined,are orthologs of the prokaryotic alpha dimer?

A)Rpb2 and rpb3
B)Rpb3 and rpb11
C)Rpb8 and rpb10
D)Rpb9 and rpb10
E)Rpb10 and rpb12

Question 9

Predict a possible effect of deleting the enhancers region of the polymerase I gene.

A)reduced transcription of ribosomes
B)reduction in the production of most hnRNAs
C)reduction in the amount of rRNA made
D)reduction in the production of Rpb1
E)reduction in the production of Rpb2

Question 10

A new mutant cell line was developed and found to be defective in polymerase III activity.Which of the following is likely to be observed in this cell line?

A)There will be an overabundance of secreted proteins.
B)Splicing function is impaired.
C)There will be an overproduction of 7 SL RNA.
D)There will be an overabundance of secreted proteins and splicing function is impaired are correct.
E)There will be an overabundance of secreted proteins,splicing function is impaired,and there will be an overproduction of 7 SL RNA are correct.

Question 11

One effect of the deletion of the hRPB1 subunit of RNA polymerase II is

A)loss of the oligosaccharide-binding domain.
B)inability of polymerase to bind to transcriptional activators.
C)slowed elongation rate.
D)non-viable cells.
E)extended CTD.

Question 12

Which of the following would you choose to study the role of polymerase subunits in elongation?

A)epitope tagging
B)immunoprecipitation
C)in vitro transcription
D)western blotting
E)DNAase footprinting

Question 13

Compared to other nuclear genes,ribosomal genes differ in their base compositions.

Question 14

Which of the following is true about the elongation complex?

A)The clamp does not come in contact with the RNA:DNA complex.
B)Processivity is slowed by the closing of the clamp.
C)The clamp closes over the RNA:DNA hybrid in the enzyme's cleft.
D)Five loops in the clamps play a role in the movement of the enzyme.
E)The active site of the enzyme lies at the end of pore 2.

Question 15

Eukaryotic ribosomal RNA genes are transcribed by RNA polymerase II.

Question 16

An antibody was produced and used in an immunohistochemical assay to detect the location of RNA polymerase I in a thin section of a liver biopsy.The section was stained with the antibody that had been labeled with a fluorescent tag,then viewed under a fluorescent microscope.State where you would expect to find positive staining if the enzyme is present.

A)rough endoplasmic reticulum
B)nucleoplasm
C)nucleolus
D)endoplasmic reticulum
E)ribosomes

Question 17

Which of the following techniques would you use to study the role of the BRE?

A)S1 nuclease protection analysis
B)linker scanning mutations
C)X-ray crystallography
D)S1 nuclease protection analysis and linker scanning mutations
E)linker scanning mutations and X-ray crystallography

Question 18

Based on studies on Saccharomyces cerevisiae,there are at least 12 subunits in RNA polymerase II.

Question 19

The CCAAT boxes are bound by which of the following?

A)Sp1
B)DPE
C)TBP
D)CTF
E)TFB

Question 20

Which of the statements about ribosomal genes is incorrect?

A)They have a higher GC content compared to other nuclear genes.
B)They have a different base composition compared to other nuclear genes.
C)There are no repetitive sequences.
D)They are found in the nucleolus.
E)They are transcribed by RNA polymerases I and III.

Question 21

Epitope tagging is an efficient way to immunoprecipate and isolate an entire polymerase complex.

Question 22

Enhancers and repressors can be tissue-specific based on the types of DNA-binding proteins that are present in the cell.

Question 23

The catalytic center of polymerase II contains Na+ ions.

Question 24

Mutational studies have shown that yeast Rpb3 is homologous to the alpha-subunit of E.coli.

Question 25

Study of yeast polymerase II has revealed a deep cleft that can accept a linear DNA template from one end to the other.

Question 26

The product of the RPB1 gene in yeast assists polymerase IIA in binding DNA.