Question 1

Which of the following would you choose to study the role of polymerase subunits in elongation?

Accepted Answer

A) epitope tagging 
B) immunoprecipitation 
C) in vitro transcription 
D) western blotting 
E) DNAase footprinting 
A) epitope tagging 
B) immunoprecipitation 
C) in vitro transcription 
D) western blotting 
E) DNAase footprinting C

Question 2

Which of the following is true about the elongation complex?

Accepted Answer

A) The clamp does not come in contact with the RNA:DNA complex. 
B) Processivity is slowed by the closing of the clamp. 
C) The clamp closes over the RNA:DNA hybrid in the enzyme's cleft. 
D) Five loops in the clamps play a role in the movement of the enzyme. 
E) The active site of the enzyme lies at the end of pore 2. 
A) The clamp does not come in contact with the RNA:DNA complex. 
B) Processivity is slowed by the closing of the clamp. 
C) The clamp closes over the RNA:DNA hybrid in the enzyme's cleft. 
D) Five loops in the clamps play a role in the movement of the enzyme. 
E) The active site of the enzyme lies at the end of pore 2. C

Question 3

Predict a possible effect of deleting the enhancers region of the polymerase I gene.

Accepted Answer

A) reduced transcription of ribosomes 
B) reduction in the production of most hnRNAs 
C) reduction in the amount of rRNA made 
D) reduction in the production of Rpb1 
E) reduction in the production of Rpb2 
A) reduced transcription of ribosomes 
B) reduction in the production of most hnRNAs 
C) reduction in the amount of rRNA made 
D) reduction in the production of Rpb1 
E) reduction in the production of Rpb2 C

Question 4

Which of the statements about ribosomal genes is incorrect?

Accepted Answer

A) They have a higher GC content compared to other nuclear genes. 
B) They have a different base composition compared to other nuclear genes. 
C) There are no repetitive sequences. 
D) They are found in the nucleolus. 
E) They are transcribed by RNA polymerases I and III. 
A) They have a higher GC content compared to other nuclear genes. 
B) They have a different base composition compared to other nuclear genes. 
C) There are no repetitive sequences. 
D) They are found in the nucleolus. 
E) They are transcribed by RNA polymerases I and III.

Question 5

Which of the following is ideal to study the function of the subunits of polymerase II?

Accepted Answer

A) Break the enzymes into component parts and gradually reassemble them. 
B) Use mutational analysis. 
C) Conduct DNA binding assays. 
D) Break the enzymes into component parts and gradually reassemble them and use mutational analysis are correct. 
E) Use mutational analysis and conduct DNA binding assays are correct. 
A) Break the enzymes into component parts and gradually reassemble them. 
B) Use mutational analysis. 
C) Conduct DNA binding assays. 
D) Break the enzymes into component parts and gradually reassemble them and use mutational analysis are correct. 
E) Use mutational analysis and conduct DNA binding assays are correct.

Question 6

The CCAAT boxes are bound by which of the following?

Accepted Answer

A) Sp1 
B) DPE 
C) TBP 
D) CTF 
E) TFB 
A) Sp1 
B) DPE 
C) TBP 
D) CTF 
E) TFB

Question 7

You were asked to choose a method to separate a mixture of eukaryotic polymerases.Which of the following methods would you choose?

Accepted Answer

A) chromatography 
B) western blotting 
C) S1 nuclease assay 
D) DNase 1 footprinting 
E) chromatography and S1 nuclease assay 
A) chromatography 
B) western blotting 
C) S1 nuclease assay 
D) DNase 1 footprinting 
E) chromatography and S1 nuclease assay

Question 8

An antibody was produced and used in an immunohistochemical assay to detect the location of RNA polymerase I in a thin section of a liver biopsy.The section was stained with the antibody that had been labeled with a fluorescent tag,then viewed under a fluorescent microscope.State where you would expect to find positive staining if the enzyme is present.

Accepted Answer

A) rough endoplasmic reticulum 
B) nucleoplasm 
C) nucleolus 
D) endoplasmic reticulum 
E) ribosomes 
A) rough endoplasmic reticulum 
B) nucleoplasm 
C) nucleolus 
D) endoplasmic reticulum 
E) ribosomes

Question 9

Based on studies on Saccharomyces cerevisiae,there are at least 12 subunits in RNA polymerase II.

Accepted Answer

A) True 
 B)False

Question 10

Which of the following techniques would you use to study the role of the BRE?

Accepted Answer

A) S1 nuclease protection analysis 
B) linker scanning mutations 
C) X-ray crystallography 
D) S1 nuclease protection analysis and linker scanning mutations 
E) linker scanning mutations and X-ray crystallography 
A) S1 nuclease protection analysis 
B) linker scanning mutations 
C) X-ray crystallography 
D) S1 nuclease protection analysis and linker scanning mutations 
E) linker scanning mutations and X-ray crystallography

Question 11

Which of the following subunits,when combined,are orthologs of the prokaryotic alpha dimer?

Accepted Answer

A) Rpb2 and rpb3 
B) Rpb3 and rpb11 
C) Rpb8 and rpb10 
D) Rpb9 and rpb10 
E) Rpb10 and rpb12 
A) Rpb2 and rpb3 
B) Rpb3 and rpb11 
C) Rpb8 and rpb10 
D) Rpb9 and rpb10 
E) Rpb10 and rpb12

Question 12

Mutational studies have shown that yeast Rpb3 is homologous to the alpha-subunit of E.coli.

Accepted Answer

A) True 
 B)False

Question 13

Compared to other nuclear genes,ribosomal genes differ in their base compositions.

Accepted Answer

A) True 
 B)False

Question 14

Enhancers and repressors can be tissue-specific based on the types of DNA-binding proteins that are present in the cell.

Accepted Answer

A) True 
 B)False

Question 15

Which of the following is involved in the stress response?

Accepted Answer

A) Rpb2 
B) Rpb3 
C) Rpb4 
D) Rpd8 
E) Rpd10 
A) Rpb2 
B) Rpb3 
C) Rpb4 
D) Rpd8 
E) Rpd10

Question 16

Study of yeast polymerase II has revealed a deep cleft that can accept a linear DNA template from one end to the other.

Accepted Answer

A) True 
 B)False

Question 17

Which of the following is not a part of the core class II promoter?

Accepted Answer

A) TATA box 
B) upstream element 
C) BRE 
D) DPE 
E) Inr 
A) TATA box 
B) upstream element 
C) BRE 
D) DPE 
E) Inr

Question 18

Eukaryotic ribosomal RNA genes are transcribed by RNA polymerase II.

Accepted Answer

A) True 
 B)False

Question 19

A new mutant cell line was developed and found to be defective in polymerase III activity.Which of the following is likely to be observed in this cell line?

Accepted Answer

A) There will be an overabundance of secreted proteins. 
B) Splicing function is impaired. 
C) There will be an overproduction of 7 SL RNA. 
D) There will be an overabundance of secreted proteins and splicing function is impaired are correct. 
E) There will be an overabundance of secreted proteins,splicing function is impaired,and there will be an overproduction of 7 SL RNA are correct. 
A) There will be an overabundance of secreted proteins. 
B) Splicing function is impaired. 
C) There will be an overproduction of 7 SL RNA. 
D) There will be an overabundance of secreted proteins and splicing function is impaired are correct. 
E) There will be an overabundance of secreted proteins,splicing function is impaired,and there will be an overproduction of 7 SL RNA are correct.

Question 20

Which of the following is true about Rpb4/7?

Accepted Answer

A) It binds to Rpb3 to facilitate melting of the DNA promoter. 
B) It extends the dock region of polymerase,making it easier for transcription factors to bind. 
C) It binds to DNA and facilitate elongation. 
D) It binds to Rpb3 to facilitate melting of the DNA promoter and it extends the dock region of polymerase,making it easier for transcription factors to bind are correct. 
E) It extends the dock region of polymerase,making it easier for transcription factors to bind and it binds to DNA and facilitate elongation are correct. 
A) It binds to Rpb3 to facilitate melting of the DNA promoter. 
B) It extends the dock region of polymerase,making it easier for transcription factors to bind. 
C) It binds to DNA and facilitate elongation. 
D) It binds to Rpb3 to facilitate melting of the DNA promoter and it extends the dock region of polymerase,making it easier for transcription factors to bind are correct. 
E) It extends the dock region of polymerase,making it easier for transcription factors to bind and it binds to DNA and facilitate elongation are correct.

Exam 10: Eukaryotic Rna Polymerases and Their Promoters

Which of the following would you choose to study the role of polymerase subunits in elongation?

Which of the following is true about the elongation complex?

Predict a possible effect of deleting the enhancers region of the polymerase I gene.

Which of the statements about ribosomal genes is incorrect?

Which of the following is ideal to study the function of the subunits of polymerase II?

The CCAAT boxes are bound by which of the following?

You were asked to choose a method to separate a mixture of eukaryotic polymerases.Which of the following methods would you choose?

Based on studies on Saccharomyces cerevisiae,there are at least 12 subunits in RNA polymerase II.

Which of the following techniques would you use to study the role of the BRE?

Which of the following subunits,when combined,are orthologs of the prokaryotic alpha dimer?

Mutational studies have shown that yeast Rpb3 is homologous to the alpha-subunit of E.coli.

Compared to other nuclear genes,ribosomal genes differ in their base compositions.

Enhancers and repressors can be tissue-specific based on the types of DNA-binding proteins that are present in the cell.

Which of the following is involved in the stress response?

Study of yeast polymerase II has revealed a deep cleft that can accept a linear DNA template from one end to the other.

Which of the following is not a part of the core class II promoter?

Eukaryotic ribosomal RNA genes are transcribed by RNA polymerase II.

A new mutant cell line was developed and found to be defective in polymerase III activity.Which of the following is likely to be observed in this cell line?

Which of the following is true about Rpb4/7?

A Brief History

The Molecular Nature of Genes

An Introduction to Gene Function

Molecular Cloning Methods

Molecular Tools for Studying Genes and Gene Activity

The Mechanism of Transcription in Bacteria

Operons: Fine Control of Bacterial Transcription

Major Shifts in Bacterial Transcription

Dna-Protein Interactions in Bacteria

General Transcription Factors in Eukaryotes

Transcription Activators in Eukaryotes

Chromatin Structure and Its Effect on Transcription

Rna Processing I: Splicing

Rna Processing II: Capping and Polyadenylation

Rna Processing: Other Post-Transcriptional Events

The Mechanism of Translation I: Initiation

The Mechanism of Translation II: Elongation and Termination

Ribosomes and Transfer Rna

Dna Replication,damage and Repair

Dna Replication II: Detailed Mechanism

Homologous Recombination

Transposition

Introduction to Genomics: Dna Sequencing on a Genomic Scale

Genomics II: Functional Genomics,proteomics and Bioinformatics

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