A 3000 Bp Circular DNA Was Treated with Both HindIII

Multiple Choice

A 3000 bp circular DNA was treated with both HindIII and EcoRI restriction enzymes.EcoRI cuts at position 100.HindIII cuts at positions 500 and 2000.Which of the following represents the fragment that would be observed if the restricted DNA were analzyed using agarose gel electrophoresis?

A) 400,100,1000,1500
B) 400,500,1500
C) 400,100,1600
D) 500,100,500,900
E) 500,1000,600