Question 1

Electroporation is commonly used to introduce recombinant DNA molecules into cells.

Accepted Answer

A) True 
 B)False  True

Question 2

Complementary DNA (cDNA) probes are produced using

Accepted Answer

A)   restriction endonucleases.
B)   RNA polymerase.
C)   DNA ligase.
D)   reverse transcriptase. D

Question 3

The enzyme reverse transcriptase was discovered by

Accepted Answer

A)   Arber and Smith.
B)   Jackson, Symons, and Berg.
C)   Boyer and Cohen.
D)   Temin and Baltimore. D

Question 4

Transposons are frequently used as cloning vectors.

Accepted Answer

A) True 
 B)False

Question 5

When a eukaryotic gene is cloned into a bacterium, the advantage of a complementary DNA (cDNA) gene being used instead of fragments of genomic DNA is that

Accepted Answer

A)   the promoter and terminator are found in the cDNA gene but not in the genomic fragment.
B)   the introns have been removed from the cDNA gene but not from the genomic fragment.
C)   the cDNA is made with the nucleotides found in the prokaryote but not in the eukaryote.
D)   There is no advantage to using a cDNA gene rather than a genomic fragment.

Question 6

When a eukaryotic gene is expressed in a bacterium, the eukaryotic regulatory sequences should be maintained in order to achieve maximum expression of the gene.

Accepted Answer

A) True 
 B)False

Question 7

Restriction endonucleases were discovered by

Accepted Answer

A)   Arber and Smith.
B)   Jackson, Symons, and Berg.
C)   Boyer and Cohen.
D)   Temin and Baltimore.

Question 8

A (n) __________ vector contains promoters that result in high-level transcription of the gene cloned within a multicloning site.

Accepted Answer

A)   shuttle
B)   chimeric
C)   expression
D)   phage

Question 9

The polymerase chain reaction (PCR) can be used to produce __________ of copies in a few hours.

Accepted Answer

A)   hundreds
B)   thousands
C)   millions
D)   billions

Question 10

Which of the following is not true of cloning vectors?

Accepted Answer

A)   They usually contain multicloning sites or polylinkers.
B)   They contain at least two replication origins.
C)   They can be replicated within an appropriate host.
D)   All of these are true of cloning vectors.

Question 11

Genetic engineering has allowed the useful production of human hemoglobin in

Accepted Answer

A)   monkeys.
B)   dogs.
C)   pigs.
D)   cows.

Question 12

The thermostable enzyme most commonly used in PCR is reverse transcriptase.

Accepted Answer

A) True 
 B)False

Question 13

Which of the following is true about restriction endonucleases?

Accepted Answer

A)   They make a blunt cut on the two DNA strands so that there are no single-strand regions.
B)   They make staggered cuts on the DNA so that single-strand ends are formed that can be used to insert foreign DNA cut with the same enzyme.
C)   Some make a blunt cut on the two DNA strands so that there are no single-strand regions and some make staggered cuts on the DNA so that single-strand ends are formed that can be used to insert foreign DNA cut with the same enzyme.
D)   Depending on the incubation conditions, the same enzyme can either make a blunt cut on the two DNA strands so that there are no single-strand regions OR make staggered cuts on the DNA so that single-strand ends are formed that can be used to insert foreign DNA cut with the same enzyme.

Question 14

Which of the following was first produced commercially using recombinant DNA technology?

Accepted Answer

A)   human growth hormone
B)   interleukins
C)   hepatitis B vaccine
D)   human insulin

Question 15

In order to express eukaryotic genes in a bacterium, the __________ must first be removed.

Accepted Answer

A)   introns
B)   exons
C)   enhancers
D)   3' poly A sequence

Question 16

Recombinant DNA technology does not rely on which of the following enzymes?

Accepted Answer

A)   restriction endonucleases
B)   RNA methylase
C)   DNA ligase
D)   reverse transcriptase

Question 17

Regardless of the exact approach taken to recombinant DNA technology, one of the keys to successful cloning is choosing the right vector.

Accepted Answer

A) True 
 B)False

Question 18

Plasmid vectors often contain __________ genes that can be used to screen for recombinants.

Accepted Answer

A)   metabolic activation
B)   antibiotic resistance
C)   insertion sequence
D)   promoter/operator

Question 19

The PCR method was developed by

Accepted Answer

A)   Boyer.
B)   Mullis.
C)   Cohen.
D)   Sanger.

Question 20

Restriction endonucleases are produced by

Accepted Answer

A)   fungi.
B)   bacteria.
C)   protozoa.
D)   plants.
E)   all of the choices

Exam 15: Recombinant Dna Technology

Electroporation is commonly used to introduce recombinant DNA molecules into cells.

Complementary DNA (cDNA) probes are produced using

The enzyme reverse transcriptase was discovered by

Transposons are frequently used as cloning vectors.

When a eukaryotic gene is cloned into a bacterium, the advantage of a complementary DNA (cDNA) gene being used instead of fragments of genomic DNA is that

When a eukaryotic gene is expressed in a bacterium, the eukaryotic regulatory sequences should be maintained in order to achieve maximum expression of the gene.

Restriction endonucleases were discovered by

A (n) __________ vector contains promoters that result in high-level transcription of the gene cloned within a multicloning site.

The polymerase chain reaction (PCR) can be used to produce __________ of copies in a few hours.

Which of the following is not true of cloning vectors?

Genetic engineering has allowed the useful production of human hemoglobin in

The thermostable enzyme most commonly used in PCR is reverse transcriptase.

Which of the following is true about restriction endonucleases?

Which of the following was first produced commercially using recombinant DNA technology?

In order to express eukaryotic genes in a bacterium, the __________ must first be removed.

Recombinant DNA technology does not rely on which of the following enzymes?

Regardless of the exact approach taken to recombinant DNA technology, one of the keys to successful cloning is choosing the right vector.

Plasmid vectors often contain __________ genes that can be used to screen for recombinants.

The PCR method was developed by

Restriction endonucleases are produced by