EcoRI Makes Staggered Cuts When It Cleaves DNA,creating Single-Stranded Tails

Multiple Choice

EcoRI makes staggered cuts when it cleaves DNA,creating single-stranded tails called "sticky ends." These ends can form a complementary base pair.Assuming that the concentration of cut DNA strands is not rate limiting in order for the complementary ends to base pair,which condition is necessary?

A) The presence of specific helicases
B) Sufficiently high temperature to dissociate complementary strands
C) Methyl groups at the end of each DNA strand
D) Sufficiently low temperature to encourage hydrogen binding of DNA strands
E) Phosphodiester bonds between the complimentary DNA strands