What Is the Major Difference Between the DNA Sequencing Techniques

Multiple Choice

What is the major difference between the DNA sequencing techniques developed by Sanger and others in the 1970s and high-throughput sequencing methods?

A) High-throughput sequencing enables the direct sequencing of a chromosome from one end to the next.
B) Unlike the earlier technology,high-throughput sequencing involves cutting the DNA into fragments.
C) Unlike the earlier technology,high-throughput sequencing relies heavily on miniaturization techniques.
D) High-throughput sequencing involves chemical modification of nucleotides,whereas the earlier technology does not.
E) Unlike the earlier technology,high-throughput sequencing does not use PCR.