You Add Substrate to an Enzyme Reaction Until the Rate

Multiple Choice

You add substrate to an enzyme reaction until the rate no longer changes,and then,upon raising the temperature,you see the reaction rate go up even further.You conclude that

A) increasing the temperature has activated an inhibitor.
B) the saturation rate was not determined at the optimal temperature.
C) the increased temperature has removed a prosthetic group,thus activating the enzyme.
D) the reaction is regulated by feedback inhibition.
E) the subunits of the enzyme have denatured.