After a Campus Outbreak of Neisseria Meningitidis (Meningococcal Serogroup B),a

Multiple Choice

After a campus outbreak of Neisseria meningitidis (meningococcal serogroup B) ,a devastating bacterial disease,which affected at least eight students at Princeton University,the US Food and Drug Administration approved the use of Bexsero to prevent the development of additional cases on that campus.Bexsero is considered to provide broader protective coverage than the US-licenced vaccines conventionally used against this disease.Which of the following methodologies was used to develop Bexsero?

A) conjugation of neisserial capsular polysaccharide to tetanus toxoid
B) reverse vaccinology
C) formalin treatment of secreted toxins
D) production of a combination vaccine that includes DTP plus a meningococcal polysaccharide diphtheria toxoid conjugate
E) engineering a nonpathogenic cattle strain of N.meningitidis to express antigens associated with pathogenic human strains.