Multiple Choice
A scientist is troubleshooting the synthesis of a cDNA library.The scientist performs both a Northern and a Southern blot.The Northern blot demonstrated the presence of RNA while the Southern blot indicated that no cDNA was present in the sample.What is likely to be the cause of the failed synthesis of the cDNA library?
A) proper primers
B) temperature too cold for annealing
C) too many dNTPs
D) defective reverse transcriptase
Correct Answer:
Verified
Correct Answer:
Verified
Q1: High throughput sequencing takes advantage of automated
Q2: A restriction enzyme that uses a six
Q3: A knock- out mouse is made with
Q5: Assume that you have cut 2 DNA
Q6: What is the main purpose of a
Q7: Why are DNA binding dyes such as
Q8: Which of the following describes a "knock-
Q9: A probe with the sequence 5'- A-
Q10: DNA ligase_ .<br>A)reconnects the bases together between
Q11: RT- PCR reverse transcription- polymerase chain reaction)can