What Is the Right Progression of Steps for a Direct-

Multiple Choice

What is the right progression of steps for a direct- sequencing experiment?

A) Lyse cells, insert genes into plasmid, purify DNA, and amplify genes by PCR.
B) Purify DNA, amplify genes by PCR, lyse cells, and insert genes into plasmid.
C) Lyse cells, insert genes into plasmid, amplify genes by PCR, and purify DNA.
D) Lyse cells, purify DNA, amplify genes by PCR, and insert genes into plasmids.