What Information Is Critical to the Success of PCR Itself

Multiple Choice

What information is critical to the success of PCR itself?

A) The complete DNA sequence of the DNA to be amplified must be known.
B) The sequence of restriction enzyme recognition sites in the DNA to be amplified and in the plasmid where the amplified DNA fragment will be cloned must be known.
C) The DNA sequence of the ends of the DNA to be amplified must be known.
D) The sequence of restriction enzyme recognition sites in the DNA to be amplified must be known.