Assume the Two E

Multiple Choice

Assume the two E.coli strains shown below are allowed to conjugate. Hfr: pro⁺, arg⁺, his⁺, lys⁺, met⁺, ampicillin- sensitive F: pro^- , arg^- , his^- , lys^- , met^- , ampicillin- resistant
What supplements would you add to glucose minimal salts agar to select for a recombinant cell that is lys⁺, arg⁺, amp- resistant?

A) ampicillin, proline, histidine, methionine
B) ampicillin, prolein, histidine, lysine
C) ampicillin, lysine, arginine
D) proline, histidine, methionine
E) lysine, arginine