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What Method Is Used to Release the N-Linked Glycan Groups β\beta

Question 53

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What method is used to release the N-linked glycan groups from the membrane-associated glycoconjugates shown in 1 in the figure below?  What method is used to release the N-linked glycan groups from the membrane-associated glycoconjugates shown in 1 in the figure below?   A)  enzymatic cleavage using PNGaseF B)  chemical cleavage using a  \beta -elimination reaction C)  chemical cleavage using a Schiff base intermediate D)  enzymatic cleavage using  \beta -galactosidase


A) enzymatic cleavage using PNGaseF
B) chemical cleavage using a β\beta -elimination reaction
C) chemical cleavage using a Schiff base intermediate
D) enzymatic cleavage using β\beta -galactosidase

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