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If Cells Are Provided with Nucleotides Labeled with a Radioactive

Question 49

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If cells are provided with nucleotides labeled with a radioactive isotope of hydrogen (3H) ,newly synthesized DNA will be radioactively labeled.In a set of experiments,a student-faculty research team introduced labeled T nucleotides into a culture of dividing human cells at specific times.Following a 30-minute incubation with the radiolabeled nucleotides,cells were lysed,and the DNA was isolated and tested for presence of radioactivity.Which of the following questions might be answered by such a method?


A) How many cells are produced by the culture per hour?
B) What is the duration of the S phase of the cell cycle?
C) What is the duration of mitosis?
D) How many picograms of DNA are contained in each nucleus?

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