A Mutation Results in an Amino Acid Substitution at a Site

Multiple Choice

A mutation results in an amino acid substitution at a site distant from the active site of an enzyme.How might this amino acid change alter the active site and substrate specificity of the enzyme?

A) by changing the optimum temperature for the enzyme
B) by changing the intracellular location of the enzyme
C) by changing the binding site for an allosteric regulator
D) by changing the conformation of the enzyme