In an Attempt to Define the Protein Domains of Protein

Essay

In an attempt to define the protein domains of protein X, you treat it with a protease and use polyacrylamide gel electrophoresis to analyze the peptides produced.In the past, you have used chymotrypsin to perform this experiment, but the stock of this enzyme has been used up.You find a stock of elastase and decide to use it instead of waiting for a new stock of chymotrypsin to arrive.
A.Give two reasons why elastase is a good substitute for chymotrypsin in this assay.
B.Why might proteolysis of the same substrate by chymotrypsin or elastase yield different results?