You are interested in the regulation of gene Q.Proteins G, H, and J are proteins that are important for regulating gene Q, and bind to its promoter region in a sequence-specific fashion.Proteins G and H both bind to site "A" but cannot bind to site "A" at the same time.Protein J binds to site "B" on the promoter.The promoter region is diagrammed in Figure 8-20. $You are interested in the regulation of gene Q.Proteins G, H, and J are proteins that are important for regulating gene Q, and bind to its promoter region in a sequence-specific fashion.Proteins G and H both bind to site A but cannot bind to site A at the same time.Protein J binds to site B on the promoter.The promoter region is diagrammed in Figure 8-20. Figure 8-20 You develop a cell-free transcriptional system to study the effects of proteins G, H, and J on the transcription of gene Q.Using this system, you can examine the effects of adding these proteins to the transcriptional system in equal amounts and measuring how much gene Q is produced.When you add these proteins to the system, you get the results shown in Table 8-20. \text { experiment }\text { reaulator protein added }\text { Q mRNA } \begin{array}{ccccc} \text { number } &\quad\quad\quad\text { G } & H & J & \quad\quad\text { made? } \\ \hline 1 &\quad\quad - & - & - & \text { no } \\ \hline 2 & \quad\quad+ & - & - & \text { no } \\ \hline 3 & \quad\quad- & + & - & \text { yes } \\ \hline 4 &\quad\quad - & - & + & \text { yes } \\ \hline 5 &\quad\quad + & + & - & \text { no } \\ \hline 6 &\quad\quad + & - & + & \text { yes } \\ \hline 7 & \quad\quad- & + & + & \text { yes } \\ \hline 8 & \quad\quad+ & + & + & \text { yes } \end{array} Table 8-20 Which proteins are likely to act as gene activators? A)G B)H C)J D)both H and J$ Figure 8-20
You develop a cell-free transcriptional system to study the effects of proteins G, H, and J on the transcription of gene Q.Using this system, you can examine the effects of adding these proteins to the transcriptional system in equal amounts and measuring how much gene Q is produced.When you add these proteins to the system, you get the results shown in Table 8-20.

\text { experiment }

\text { reaulator protein added }

\text { Q mRNA }

\begin{array}{ccccc}\text { number } &\quad\quad\quad\text { G } & H & J & \quad\quad\text { made? } \\\hline 1 &\quad\quad - & - & - & \text { no } \\\hline 2 & \quad\quad+ & - & - & \text { no } \\\hline 3 & \quad\quad- & + & - & \text { yes } \\\hline 4 &\quad\quad - & - & + & \text { yes } \\\hline 5 &\quad\quad + & + & - & \text { no } \\\hline 6 &\quad\quad + & - & + & \text { yes } \\\hline 7 & \quad\quad- & + & + & \text { yes } \\\hline 8 & \quad\quad+ & + & + & \text { yes }\end{array}

Table 8-20
Which proteins are likely to act as gene activators?
A)G
B)H
C)J
D)both H and J

From the Sequencing of the Human Genome, We Believe That

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