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With Fully Automated Sanger Sequencing, All Four Chain-Terminating DdNTPs Are

Question 39

Multiple Choice

With fully automated Sanger sequencing, all four chain-terminating ddNTPs are added into a single reaction and loaded onto a capillary gel.The sequence can be determined even though all ddNTPs are mixed together because


A) the Sanger sequencing reactions do not contain dNTPs and use the ddNTPS to synthesize the DNA.
B) the Sanger sequencing reactions utilize ddNTPs, each labeled with a different fluorescent tag, which allows all four ddNTPs to be incorporated into a single molecule of DNA.
C) the Sanger sequencing reactions generate a set of products, each of which carries a single fluorescent tag whose color reveals the identity of the base that is at the end of the product.
D) the fully automated Sanger sequencing reactions do not require DNA polymerase because the bases are read as the DNA is pulled through a tiny pore at the end of the capillary gel.

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