In Step 2 of the Citric Acid Cycle, the Enzyme

Multiple Choice

In step 2 of the citric acid cycle, the enzyme aconitase generates isocitrate from citrate.Which of the following statements about this reaction is TRUE?

A) There is a substantial free-energy difference between the reactants and products of this reaction.
B) The unbonded electrons from hydroxide ions provide energy for this reaction.
C) The aconitase enzyme functions as a mutase in this reaction.
D) The reaction sequence first generates one molecule of water and then consumes one molecule of water.