Cells Have Oligosaccharides Displayed on Their Cell Surface That Are

Multiple Choice

Cells have oligosaccharides displayed on their cell surface that are important for cell-cell recognition.Your friend discovered a transmembrane glycoprotein, GP1, on a pathogenic yeast cell that is recognized by human immune cells.He decides to purify large amounts of GP1 by expressing it in bacteria.To his purified protein he then adds a branched 14-sugar oligosaccharide to the asparagine of the only Asn-X-Ser sequence found on GP1 (Figure 15-32) .Unfortunately, immune cells do not seem to recognize this synthesized glycoprotein.Which of the following statements is a likely explanation for this problem? Figure 15-32

A) The oligosaccharide should have been added to the serine instead of the asparagine.
B) The oligosaccharide should have been added one sugar at a time.
C) The oligosaccharide needs to be further modified before it is mature.
D) The oligosaccharide needs a disulfide bond.