You Have Discovered a Gene That Appears to Be Crucial

Multiple Choice

You have discovered a gene that appears to be crucial to the development of the nervous system in C.elegans.However,you have been unsuccessful in creating embryos in which this gene is knocked out.How might you study the function of this gene in C.elegans without creating a "knockout" mutant?

A) CRISPR/Cas targeted gene disruption
B) RNAi gene knockdown
C) transcriptional activator-like effector nucleases (TALENs) targeted gene disruption
D) next-generation sequencing.
E) restriction endonuclease mapping.