Using the Techniques of Genetic Engineering,you Design a Protein You

Multiple Choice

Using the techniques of genetic engineering,you design a protein you want to accumulate within the ER of yeast cells.To accomplish this goal,you need to

A) do nothing; all proteins go through the ER.
B) incorporate appropriate mannose-6-phosphate groups.
C) incorporate the appropriate DNA sequence(s) to create signal sequences in the mature peptide.
D) incorporate radioactive amino acids into the protein.
E) remove the N-formyl group located at the N-terminus of the polypeptide.