Although MCF-10A Breast Cancer Cells Grow Well on Matrigel-Coated Culture

Multiple Choice

Although MCF-10A breast cancer cells grow well on Matrigel-coated culture dishes,the cells will form cysts of polarized cells that produce milk only when

A) extra collagen is added to the Matrigel.
B) extra fibronectin is added to the Matrgel.
C) extra elastin is added to the Matrigel.
D) the Matrigel layer is thick enough to form a 3D environment.
E) the Matrigel layer is present as a very thin layer on the culture dishes.