You Are Interested in the Mobility of Two Different Unknown

Essay

You are interested in the mobility of two different unknown integral plasma membrane proteins-protein X and protein Y-in a cell.In one cell,you fluorescently label all the X proteins.In another cell,you label all the Y proteins.When you perform FRAP analysis on both cells,you find that most of the protein X fluorescence has recovered within 3 minutes,but recovery of the protein Y fluorescence is significantly slower.Even after 10 minutes,only 50% of the protein Y fluorescence has recovered.Describe the FRAP experimental protocol,explain what the results tell you about mobility of the X and Y proteins,and propose a possible explanation for why the mobilities of the two proteins differ.