Multiple Choice
Some researchers have hypothesized that type II diabetes mellitus is caused by a mitochondrial defect.Which of the following would be the best way for them to study mitochondrial differences in fresh tissue samples from diabetic and nondiabetic mice?
A) Mitochondria could be identified and isolated from other cellular components using immunofluorescence microscopy in both tissues.
B) Mitochondria are the only organelles that survive tissue homogenization,thus both tissues could be homogenized to study the mitochondria.
C) Mitochondria from both tissues could be isolated from other cellular components by using proteolytic enzymes to break down other cellular structures.
D) Mitochondria from both tissues could be isolated from other cellular components by fractionating tissue homogenate using differential centrifugation followed by density gradient centrifugation.
E) Mitochondria can not be separated from whole cells without damaging them.
Correct Answer:
Verified
Correct Answer:
Verified
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