If the Tn10 Transposon Is Used to Mutagenize a Bacterium

Multiple Choice

If the Tn10 transposon is used to mutagenize a bacterium for the purpose of creating a mutation in the his operon, what would be the most appropriate selection media?

A) rich media that contains all of the amino acids supplemented with tetracycline
B) minimal media supplemented only with histidine
C) minimal media supplemented with tetracycline and histidine
D) minimal media supplemented with tetracycline alone