Your Research Project Involves the Characterization of a Recently Identified

Multiple Choice

Your research project involves the characterization of a recently identified transcription factor.As part of your project,you want to determine if this transcription factor binds directly to any of the general transcription factors.Unfortunately,you are having trouble expressing the full-length protein.What approach may help achieve your research goal?

A) You must find a way to express the full-length protein,since transcription factor functions do not lie in separate domains.
B) Identify the RNA that mediates the protein-protein binding.
C) Express and purify only the DNA-binding domain,and test for binding.
D) Express and purify only the activation domain,and test for binding.