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Which of the Following Is NOT True of Bisulfite Sequencing

Question 31

Multiple Choice

Which of the following is NOT true of bisulfite sequencing?


A) Methylated cytosines are unaffected by sodium bisulfite.
B) DNA is treated with sodium bisulfite, which chemically converts unmethylated cytosines to uracil.
C) Unmethylated cytosines are detected as thymine during sequencing.
D) All thymines revealed in DNA sequencing of bisulfite treated DNA were cytosines in the original, untreated DNA.
E) The sequence of genomic DNA treated with sodium bisulfite is compared to the sequence of untreated genomic DNA.

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