You are attempting to generate a physical map of the bacteriophage

\lambda

genome using restriction enzyme digestions. You digest the phage DNA with EcoRI or NcoI or both, and observe the fragment sizes listed below. You know that bacteriophage

\lambda

has a linear, not circular, genome.

\begin{array} { l l l } { \text { EcoRI } } & { \text { NcoI } } & { \text { EcoRI/NcoI } } \\\hline { 21.2 \mathrm {~kb} }&{ 19.3 \mathrm {~kb} } &{ 19.3 \mathrm {~kb} } \\7.4 \mathrm {~kb} & 16.4 \mathrm {~kb} & 7.4 \mathrm {~kb} \\5.8 \mathrm {~kb} & 4.6 \mathrm {~kb} & 5.1 \mathrm {~kb} \\5.7 \mathrm {~kb} & 4.2 \mathrm {~kb} & 3.9 \mathrm {~kb} \\4.9 \mathrm {~kb} & 4.0 \mathrm {~kb} & 3.5 \mathrm {~kb} \\3.5 \mathrm {~kb} & & 2.7 \mathrm {~kb} \\& & 2.2 \mathrm {~kb} \\& & 1.9 \mathrm {~kb} \\& & 1.8 \mathrm {~kb} \\& & 0.7 \mathrm {~kb}\end{array}

a. How many times does the EcoRI enzyme cut the

\lambda

DNA? How many times does the NcoI enzyme cut the

\lambda

DNA?
b. Use the fragment size data to generate a physical map of the bacteriophage

\lambda

genome, indicating the order and relative positions of the restriction enzyme recognition sites.

Which of the Following Is Involved in the RNA Sequencing

Multiple Choice

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